Identification of CIC-2-like chloride currents in pig pancreatic acinar cells

Carew, M. A. and Thorn, P. (1996) Identification of CIC-2-like chloride currents in pig pancreatic acinar cells. Pfluegers Archiv: European Journal of Physiology, 433 1-2: 84-90. doi:10.1007/s004240050252


Author Carew, M. A.
Thorn, P.
Title Identification of CIC-2-like chloride currents in pig pancreatic acinar cells
Journal name Pfluegers Archiv: European Journal of Physiology   Check publisher's open access policy
ISSN 0031-6768
Publication date 1996-11-01
Year available 1996
Sub-type Article (original research)
DOI 10.1007/s004240050252
Open Access Status Not yet assessed
Volume 433
Issue 1-2
Start page 84
End page 90
Total pages 7
Place of publication Heidelberg, Germany
Publisher Springer
Language eng
Abstract We used the whole-cell patch-clamp technique to identify a hyperpolarization-activated Cl- current (approximate to 50 pA/pF at -60 mV) in acutely isolated, voltage-clamped, single, pig pancreatic acinar cells. This current had characteristic properties of inward rectification, a Cl- = Br->I- selectivity sequence and activation by extracellular hypotonicity. These properties are similar to those reported for the ClC-2 Cl- channel recently cloned from rat and expressed in oocytes. An antiserum raised against the C-terminus of ClC-2 localized the channel to secretory granules containing amylase that were situated exclusively at the apical pole of the pig pancreatic acinar cells, but the channel was not localized in the basolateral membrane. Our study combines a functional assessment and immunohistochemical localization of ClC-2-like channels in a native mammalian cell. The data suggest that the ClC-2-like Cl- channel may function as a Cl- efflux pathway in pancreatic acinar cells.
Keyword Chloride channels
Pancreatic acinar cells
CIC-2
Volume-sensitivity
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biomedical Sciences Publications
 
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Created: Thu, 10 Mar 2011, 18:45:16 EST