Cutting Edge: Mincle is essential for recognition and adjuvanticity of the mycobacterial cord factor and its synthetic analog trehalose-dibehenate

Schoenen, Hanne, Bodendorfer, Barbara, Hitchens, Kelly, Manzanero, Silvia, Werninghaus, Kerstin, Nimmerjahn, Falk, Agger, Else Marie, Stenger, Steffen, Andersen, Peter, Ruland, Jürgen, Brown, Gordon D., Wells, Christine and Lang, Roland (2010) Cutting Edge: Mincle is essential for recognition and adjuvanticity of the mycobacterial cord factor and its synthetic analog trehalose-dibehenate. Journal of Immunology, 184 6: 2756-2760. doi:10.4049/jimmunol.0904013


Author Schoenen, Hanne
Bodendorfer, Barbara
Hitchens, Kelly
Manzanero, Silvia
Werninghaus, Kerstin
Nimmerjahn, Falk
Agger, Else Marie
Stenger, Steffen
Andersen, Peter
Ruland, Jürgen
Brown, Gordon D.
Wells, Christine
Lang, Roland
Title Cutting Edge: Mincle is essential for recognition and adjuvanticity of the mycobacterial cord factor and its synthetic analog trehalose-dibehenate
Journal name Journal of Immunology   Check publisher's open access policy
ISSN 0022-1767
1550-6606
Publication date 2010-03-01
Year available 2010
Sub-type Article (original research)
DOI 10.4049/jimmunol.0904013
Open Access Status Not yet assessed
Volume 184
Issue 6
Start page 2756
End page 2760
Total pages 5
Place of publication Bethesda, MD, U.S.A.
Publisher American Association of Immunologists
Language eng
Abstract The mycobacterial cord factor trehalose-6,6-dimycolate (TDM) and its synthetic analog trehalose-6,6-dibehenate (TDB) are potent adjuvants for Th1/Th17 vaccination that activate Syk-Card9 signaling in APCs. In this study, we have further investigated the molecular mechanism of innate immune activation by TDM and TDB. The Syk-coupling adapter protein FcR gamma was essential for macrophage activation and Th17 adjuvanticity. The FcR gamma-associated C-type lectin receptor Mincle was expressed in macrophages and upregulated by TDM and TDB. Recombinant Mincle-Fc fusion protein specifically bound to the glycolipids. Genetic ablation or Mincle abolished TDM/TDB-induced macrophage activation and induction of T cell immune responses to a tuberculosis subunit vaccine. Macrophages lacking Mincle or FcR gamma were impaired in the inflammatory response to Mycobacterium bovis bacillus Calmette-Guerin. These results establish that Mincle is a key receptor for the mycobacterial cord factor and controls the Th1/Th17 adjuvanticity of TDM and TDB. The Journal of Immunology, 2010, 184: 2756-2760.
Keyword Immunology
Immunology
IMMUNOLOGY
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID SFB796
LSHP-CT-2003-503367
455947
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
Australian Institute for Bioengineering and Nanotechnology Publications
 
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Created: Thu, 10 Mar 2011, 00:34:51 EST