Structure analysis of 16S rDNA sequences from strains of Acidithiobacillus ferrooxidans

Peng, H., Yang, Y., Li, X., Qiu, G. Z., Liu, X. D., Huang, J. F. and Hu, Y. H. (2006) Structure analysis of 16S rDNA sequences from strains of Acidithiobacillus ferrooxidans. Journal of Biochemistry And Molecular Biology, 39 2: 178-182.

Author Peng, H.
Yang, Y.
Li, X.
Qiu, G. Z.
Liu, X. D.
Huang, J. F.
Hu, Y. H.
Title Structure analysis of 16S rDNA sequences from strains of Acidithiobacillus ferrooxidans
Journal name Journal of Biochemistry And Molecular Biology   Check publisher's open access policy
ISSN 1225-8687
Publication date 2006-03-01
Year available 2006
Sub-type Article (original research)
Open Access Status Not yet assessed
Volume 39
Issue 2
Start page 178
End page 182
Total pages 5
Place of publication Seoul, Korea
Publisher Korean Society for Biochemistry and Molecular Biology
Language eng
Abstract Four strains of Acidithiobacillus ferrooxidans with different iron oxidation capacity were isolated from different mine drainage stations. The 16S rRNA gene of these strains were cloned and sequenced. Based on our sequences analysis on the four strain and the data on the other strains deposited in Genbank, all A. ferrooxidans may be classified into three phylogenetic groups. The analysis data showed that nucleotide variables (signature sites) were detected in 21 positions, and most of them were found in the first 800bp from 5' terminal except position 970 and 1375. Interestingly, the first 13 signature sites were located in two main regions: the first region (position 175-234) located in V2 while the second region (position 390-439) were detected in constant region between V2 and V3. Furthermore, the secondary structure and minimal free energy were determined in two regions among strains of three groups. These results may be useful in characterizing the microevolutionary mechanisms of species formation and monitoring in biohydrometallurgical application.
Keyword Acidithiobacillus ferrooxidans
16S rDNA sequence
secondary structure
signature site
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biomedical Sciences Publications
 
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Created: Wed, 09 Mar 2011, 23:08:49 EST