Yeast genome-wide expression analysis identifies a strong ergosterol and oxidative stress response during the initial stages of an industrial lager fermentation

Higgins, Vincent J., Beckhouse, Anthony G., Oliver, Anthony D., Rogers, Peter J. and Dawes, Ian W. (2003) Yeast genome-wide expression analysis identifies a strong ergosterol and oxidative stress response during the initial stages of an industrial lager fermentation. Applied And Environmental Microbiology, 69 8: 4777-4787. doi:10.1128/AEM.69.8.4777-4787.2003

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Author Higgins, Vincent J.
Beckhouse, Anthony G.
Oliver, Anthony D.
Rogers, Peter J.
Dawes, Ian W.
Title Yeast genome-wide expression analysis identifies a strong ergosterol and oxidative stress response during the initial stages of an industrial lager fermentation
Journal name Applied And Environmental Microbiology   Check publisher's open access policy
ISSN 0099-2240
1098-5336
Publication date 2003-08-01
Sub-type Article (original research)
DOI 10.1128/AEM.69.8.4777-4787.2003
Open Access Status File (Publisher version)
Volume 69
Issue 8
Start page 4777
End page 4787
Total pages 11
Place of publication Washington, DC United States
Publisher American Society for Microbiology
Language eng
Formatted abstract
Genome-wide expression analysis of an industrial strain of Saccharomyces cerevisiae during the initial stages of an industrial lager fermentation identified a strong response from genes involved in the biosynthesis of ergosterol and oxidative stress protection. The induction of the ERG genes was confirmed by Northern analysis and was found to be complemented by a rapid accumulation of ergosterol over the initial 6-h fermentation period. From a test of the metabolic activity of deletion mutants in the ergosterol biosynthesis pathway, it was found that ergosterol is an important factor in restoring the fermentative capacity of the cell after storage. Additionally, similar ERG10 and TRR1 gene expression patterns over the initial 24-h fermentation period highlighted a possible interaction between ergosterol biosynthesis and the oxidative stress response. Further analysis showed that erg mutants producing altered sterols were highly sensitive to oxidative stress-generating compounds. Here we show that genome-wide expression analysis can be used in the commercial environment and was successful in identifying environmental conditions that are important in industrial yeast fermentation.
Keyword Biosynthesis
Fermentation
Genes
Yeast
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
 
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Created: Wed, 09 Mar 2011, 18:15:49 EST