Fluorescence depolarization and quenching studies of acenaphthalene-labelled poly(acrylamide) in water

Pascal, Philippe, Duhamel, Jean, Wang, Yongcai, Winnik, Mitchell A., Zhu, Xiao Xia, Macdonald, Peter, Napper, Donald H. and Gilbert, Robert G. (1993) Fluorescence depolarization and quenching studies of acenaphthalene-labelled poly(acrylamide) in water. Polymer, 34 6: 1134-1140. doi:10.1016/0032-3861(93)90763-Z


Author Pascal, Philippe
Duhamel, Jean
Wang, Yongcai
Winnik, Mitchell A.
Zhu, Xiao Xia
Macdonald, Peter
Napper, Donald H.
Gilbert, Robert G.
Title Fluorescence depolarization and quenching studies of acenaphthalene-labelled poly(acrylamide) in water
Journal name Polymer   Check publisher's open access policy
ISSN 0032-3861
1873-2291
Publication date 1993-03-01
Sub-type Article (original research)
DOI 10.1016/0032-3861(93)90763-Z
Volume 34
Issue 6
Start page 1134
End page 1140
Total pages 7
Place of publication Oxford, United Kingdom
Publisher Elsevier
Language eng
Formatted abstract
Acenaphthalene was copolymerized with acrylamide in water to form poly(acrylamide-co-acenaphthalene) [P(AAm-co-ACE)] with molecular weight ≈2 × 10 6 g mol -1. A variety of fluorescence experiments were carried out at 0.8 wt% polymer concentration. Time-resolved fluorescence depolarization measurements gave a mean rotational correlation time for backbone motion of 30 ns. Time-resolved and steady-state fluorescence quenching experiments were carried out using acrylamide as the quencher. In the case of polymer-bound chromophore, we were able to follow the transient effect in the diffusion-controlled quenching process. The mutual diffusion coefficient D characterizing this reaction is a factor of six slower than the self-diffusion coefficient D AAm = 9.15 × 10 -6 cm 2 s -1 for acrylamide in water, measured by pulsed gradient n.m.r. We interpret this result to mean that acrylamide diffusion is significantly slower inside the P(AAm) coil than it is in bulk water.
Keyword Depolarization
Fluorescence
Poly(acrylamide)
Energy-transfer
Transient
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Chemistry and Molecular Biosciences
Centre for Nutrition and Food Sciences Publications
 
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Created: Tue, 08 Mar 2011, 01:54:19 EST