Asymmetric cell division of T cells upon antigen presentation uses multiple conserved mechanisms

Oliaro, Jane, Van Ham, Vanessa, Sacirbegovic, Faruk, Pasam, Anupama, Bomzon, Ze'ev, Kim Pham, Ludford-Menting, Mandy J., Waterhouse, Nigel J., Bots, Michael, Hawkins, Edwin D., Watt, Sally V., Cluse, Leonie A., Clarke, Chris J. P., Izon, David J., Chang, John T., Thompson, Natalie, Gu, Min, Johnstone, Ricky W., Smyth, Mark J., Humbert, Patrick O., Reiner, Steven L. and Russell, Sarah M. (2010) Asymmetric cell division of T cells upon antigen presentation uses multiple conserved mechanisms. The Journal of Immunology, 185 1: 367-375. doi:10.4049/jimmunol.0903627

Author Oliaro, Jane
Van Ham, Vanessa
Sacirbegovic, Faruk
Pasam, Anupama
Bomzon, Ze'ev
Kim Pham
Ludford-Menting, Mandy J.
Waterhouse, Nigel J.
Bots, Michael
Hawkins, Edwin D.
Watt, Sally V.
Cluse, Leonie A.
Clarke, Chris J. P.
Izon, David J.
Chang, John T.
Thompson, Natalie
Gu, Min
Johnstone, Ricky W.
Smyth, Mark J.
Humbert, Patrick O.
Reiner, Steven L.
Russell, Sarah M.
Title Asymmetric cell division of T cells upon antigen presentation uses multiple conserved mechanisms
Journal name The Journal of Immunology   Check publisher's open access policy
ISSN 0022-1767
Publication date 2010-07-01
Sub-type Article (original research)
DOI 10.4049/jimmunol.0903627
Open Access Status Not yet assessed
Volume 185
Issue 1
Start page 367
End page 375
Total pages 9
Place of publication Bethesda, MD, United States
Publisher American Association of Immunologists
Language eng
Formatted abstract
Asymmetric cell division is a potential means by which cell fate choices during an immune response are orchestrated. Defining the molecular mechanisms that underlie asymmetric division of T cells is paramount for determining the role of this process in the generation of effector and memory T cell subsets. In other cell types, asymmetric cell division is regulated by conserved polarity protein complexes that control the localization of cell fate determinants and spindle orientation during division.We have developed a tractable, in vitro model of naive CD8+ T cells undergoing initial division while attached to dendritic cells during Ag presentation to investigate whether similar mechanisms might regulate asymmetric division of T cells. Using this system, we show that direct interactions with APCs provide the cue for polarization of T cells. Interestingly, the immunological synapse disseminates before division even though the T cells retain contact with the APC. The cue from the APC is translated into polarization of cell fate determinants via the polarity network of the Par3 and Scribble complexes, and orientation of the mitotic spindle during division is orchestrated by the partner of inscuteable/G protein complex. These findings suggest that T cells have selectively adapted a number of evolutionarily conserved mechanisms to generate diversity through asymmetric cell division.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
School of Medicine Publications
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Created: Tue, 08 Mar 2011, 01:16:32 EST by Debbie Banks on behalf of School of Medicine