Stem cell integrins: Implications for ex-vivo culture and cellular therapies

Prowse, Andrew B. J., Chong, Fenny, Gray, Peter P. and Munro, Trent P. (2011) Stem cell integrins: Implications for ex-vivo culture and cellular therapies. Stem Cell Research, 6 1: 1-12. doi:10.1016/j.scr.2010.09.005

Author Prowse, Andrew B. J.
Chong, Fenny
Gray, Peter P.
Munro, Trent P.
Title Stem cell integrins: Implications for ex-vivo culture and cellular therapies
Journal name Stem Cell Research   Check publisher's open access policy
ISSN 1873-5061
Publication date 2011-01-01
Year available 2010
Sub-type Critical review of research, literature review, critical commentary
DOI 10.1016/j.scr.2010.09.005
Open Access Status DOI
Volume 6
Issue 1
Start page 1
End page 12
Total pages 12
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Formatted abstract
Use of stem cells, whether adult or embryonic for clinical applications to treat diseases such as Parkinson's, macular degeneration or Type I diabetes will require a homogenous population of mature, terminally differentiated cells. A current area of intense interest is the development of defined surfaces for stem cell derivation, maintenance, proliferation and subsequent differentiation, which are capable of replicating the complex cellular environment existing in vivo. During development many cellular cues result from integrin signalling induced by the local extracellular matrix. There are 24 known integrin heterodimers comprised of one of 18 α subunits and one of 8 β subunits and these have a diverse range of functions mediating cell-cell adhesion, growth factor receptor responses and intracellular signalling cascades for cell migration, differentiation, survival and proliferation. We discuss here a brief summary of defined conditions for human embryonic stem cell culture together with a description of integrin function and signalling pathways. The importance of integrin expression during development is highlighted as critical for lineage specific cell function and how consideration of the integrin expression profile should be made while differentiating stem cells for use in therapy. In addition this review summarises the known integrin expression profiles for human embryonic stem cells and 3 common adult stem cell types: mesenchymal, haematopoietic and neural. We then outline some of the possible technologies available for investigating cell-extracellular matrix interactions and subsequent integrin mediated cell responses.
Keyword Human embryonic stem
Human bone-marrow
Term self-renewal
Hematopoietic stem
Chondrogenic differentiation
Adhesion molecules
Progenitor cells
Fetal liver
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online 7 October 2010.

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collections: Official 2012 Collection
Australian Institute for Bioengineering and Nanotechnology Publications
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Citation counts: TR Web of Science Citation Count  Cited 65 times in Thomson Reuters Web of Science Article | Citations
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Created: Sun, 06 Mar 2011, 10:01:45 EST