The roles of apoptotic pathways in the low recovery rate after cryopreservation of dissociated human embryonic stem cells

Xu, Xia, Cowley, Sally, Flaim, Christopher J., James, William, Seymour, Leonard and Cui, Zhanfeng (2010) The roles of apoptotic pathways in the low recovery rate after cryopreservation of dissociated human embryonic stem cells. Biotechnology Progress, 26 3: 827-837. doi:10.1002/btpr.368


Author Xu, Xia
Cowley, Sally
Flaim, Christopher J.
James, William
Seymour, Leonard
Cui, Zhanfeng
Title The roles of apoptotic pathways in the low recovery rate after cryopreservation of dissociated human embryonic stem cells
Journal name Biotechnology Progress   Check publisher's open access policy
ISSN 8756-7938
1520-6033
Publication date 2010-05-01
Sub-type Article (original research)
DOI 10.1002/btpr.368
Open Access Status DOI
Volume 26
Issue 3
Start page 827
End page 837
Total pages 11
Place of publication New York, NY, U.S.A.
Publisher John Wiley for the American Institute of Chemical Engineers
Language eng
Formatted abstract
Human embryonic stem (hES) cells have enormous potential for clinical applications. However, one major challenge is to achieve high cell recovery rate after cryopreservation. Understanding how the conventional cryopreservation protocol fails to protect the cells is a prerequisite for developing efficient and successful cryopreservation methods for hES cell lines and banks. We investigated how the stimuli from cryopreservation result in apoptosis, which causes the low cell recovery rate after cryopreservation. The level of reactive oxygen species (ROS) is significantly increased, F-actin content and distribution is altered, and caspase-8 and caspase-9 are activated after cryopreservation. p53 is also activated and translocated into nucleus. During cryopreservation apoptosis is induced by activation of both caspase-8 through the extrinsic pathway and caspase-9 through the intrinsic pathway. However, exactly how the extrinsic pathway is activated is still unclear and deserves further investigation.
© 2009, 2010 American Institute of Chemical Engineers
Keyword Cryopreservation of hES cells
Apoptosis pathways
Reactive oxygen species
F-actin
Apoptosis inhibitors
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ
Additional Notes Issue published May/June 2010.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
Australian Institute for Bioengineering and Nanotechnology Publications
 
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