Human fetal mesenchymal stem cells differentiate into brown and white adipocytes: a role for ERRalpha in human UCP1 expression

Morganstein, Daniel L., Wu, Pensee, Mane, Meritxell R., Fisk, Nick M., White, Roger and Parker, Malcolm G. (2010) Human fetal mesenchymal stem cells differentiate into brown and white adipocytes: a role for ERRalpha in human UCP1 expression. Cell Research, 20 4: 434-444. doi:10.1038/cr.2010.11

Attached Files (Some files may be inaccessible until you login with your UQ eSpace credentials)
Name Description MIMEType Size Downloads
Fisk_authaffil_staffdata.pdf HERDC evidence – not publicly available application/pdf 85KB 0

Author Morganstein, Daniel L.
Wu, Pensee
Mane, Meritxell R.
Fisk, Nick M.
White, Roger
Parker, Malcolm G.
Title Human fetal mesenchymal stem cells differentiate into brown and white adipocytes: a role for ERRalpha in human UCP1 expression
Formatted title
Human fetal mesenchymal stem cells differentiate into brown and white adipocytes: a role for ERRα in human UCP1 expression
Journal name Cell Research   Check publisher's open access policy
ISSN 1001-0602
Publication date 2010-04-01
Sub-type Article (original research)
DOI 10.1038/cr.2010.11
Open Access Status Not Open Access
Volume 20
Issue 4
Start page 434
End page 444
Total pages 11
Place of publication United Kingdom
Publisher Nature Publishing Group
Language eng
Abstract We investigated the ability of fetal mesenchymal stem cells (fMSCs) to differentiate into brown and white adipocytes and compared the expression of a number of marker genes and key regulatory factors. We showed that the expression of key adipocyte regulators and markers during differentiation is similar to that in other human and murine adipocyte models, including induction of PPARγ2 and FABP4. Notably, we found that the preadipocyte marker, Pref-1, is induced early in differentiation and then declines markedly as the process continues, suggesting that fMSCs first acquire preadipocyte characteristics as they commit to the adipogenic lineage, prior to their differentiation into mature adipocytes. After adipogenic induction, some stem cell isolates differentiated into cells resembling brown adipocytes and others into white adipocytes. Detailed investigation of one isolate showed that the novel brown fat-determining factor PRDM16 is expressed both before and after differentiation. Importantly, these cells exhibited elevated basal UCP-1 expression, which was dependent on the activity of the orphan nuclear receptor ERRα, highlighting a novel role for ERRα in human brown fat. Thus fMSCs represent a useful in vitro model for human adipogenesis, and provide opportunities to study the stages prior to commitment to the adipocyte lineage. They also offer invaluable insights into the characteristics of human brown fat. © 2010 IBCB, SIBS, CAS All rights reserved.
Keyword Adipocytes
Adipogenesis
Brown
ERRα
Mesenchymal stem cells
Prdm16
Ucp1
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2011 Collection
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 36 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 41 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Mon, 21 Feb 2011, 23:42:43 EST by Caroline Irle on behalf of UQ Centre for Clinical Research