Matrix matching in liquid chromatography-mass spectrometry with stable isotope labelled internal standards - Is it necessary?

Hewavitharana, Amitha K. (2011) Matrix matching in liquid chromatography-mass spectrometry with stable isotope labelled internal standards - Is it necessary?. Journal of Chromatography A, 1218 2: 359-361. doi:10.1016/j.chroma.2010.11.047


Author Hewavitharana, Amitha K.
Title Matrix matching in liquid chromatography-mass spectrometry with stable isotope labelled internal standards - Is it necessary?
Journal name Journal of Chromatography A   Check publisher's open access policy
ISSN 0021-9673
1873-3778
Publication date 2011-01-14
Year available 2010
Sub-type Article (original research)
DOI 10.1016/j.chroma.2010.11.047
Open Access Status Not yet assessed
Volume 1218
Issue 2
Start page 359
End page 361
Total pages 3
Place of publication Amsterdam, Netherlands
Publisher Elsevier BV
Language eng
Subject 1602 Analytical Chemistry
1605 Organic Chemistry
1303 Biochemistry
Abstract Matrix matching is used in analysis to compensate for matrix effects that influence analytical response. It has been a widely discussed topic in electro-spray mass spectrometry where the ionization suppression is a major problem in accurate quantitative analysis. However, the unique strength of mass spectrometry to detect and quantify accurately a co-eluting stable isotope labelled internal standard offers an easy solution to the ionization suppression problem. Given the fact that it is impossible to match the matrix of the calibration standards with all samples, mass spectrometry allows accurate quantitation without the need for matrix matching, as long as the internal standard co-elutes with the analyte of interest. If the analyte and internal standard co-elute, the slope of the calibration curve analyte response/internal standard vs. analyte concentration is independent of the matrix composition, eliminating the need for matrix matching. © 2010 Elsevier B.V.
Keyword Matrix effects
Stable isotope labelled internal standard
Ion Suppression
Electro-spray MS
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published under Short Communications. Available online 27 November 2010.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Pharmacy Publications
 
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Created: Tue, 15 Feb 2011, 21:21:41 EST by Charna Kovacevic on behalf of School of Pharmacy