The effect of estradiol on COX-2, EP2, and EP4 mRNA expression and the extracellular matrix in the cervix of the hypogonadotrophic, ovariectomized ewe

Kershaw-Young, C. M., Scaramuzzi, R. J., McGowan, M. R., Pitsillides, A. A., Wheeler-Jones, C. P. D. and Khalid, M. (2010) The effect of estradiol on COX-2, EP2, and EP4 mRNA expression and the extracellular matrix in the cervix of the hypogonadotrophic, ovariectomized ewe. Theriogenology, 73 5: 620-628. doi:10.1016/j.theriogenology.2009.10.018


Author Kershaw-Young, C. M.
Scaramuzzi, R. J.
McGowan, M. R.
Pitsillides, A. A.
Wheeler-Jones, C. P. D.
Khalid, M.
Title The effect of estradiol on COX-2, EP2, and EP4 mRNA expression and the extracellular matrix in the cervix of the hypogonadotrophic, ovariectomized ewe
Formatted title
The effect of estradiol on COX-2, EP2, and EP4 mRNA expression and the extracellular matrix in the cervix of the hypogonadotrophic, ovariectomized ewe
Journal name Theriogenology   Check publisher's open access policy
ISSN 0093-691X
1879-3231
Publication date 2010-03-15
Year available 2009
Sub-type Article (original research)
DOI 10.1016/j.theriogenology.2009.10.018
Open Access Status Not yet assessed
Volume 73
Issue 5
Start page 620
End page 628
Total pages 9
Editor F. Gandolfi
J. Kastelic
Place of publication Philadelphia, PA, U.S.A.
Publisher Elsevier
Language eng
Formatted abstract
There is a degree of cervical relaxation in the ewe at estrus that is regulated by changes in prostaglandin synthesis, prostaglandin receptor expression, and changes in the cervical extracellular matrix. It is likely that these are regulated by changes in periovulatory hormones, particularly estradiol. This study determined the effect of estradiol benzoate on the mRNA expression of cyclooxygenase-2 (COX-2) and the prostaglandin E receptors EP2 and EP4, the concentration of cervical hyaluronan, and the proportion of smooth muscle and collagen in the cervix of the hypogonadotrophic ovariectomized ewe (Ovis aries). Ovariectomized hypogonadotrophic ewes were given 100 μg estradiol benzoate, and their cervices were collected 0, 24, and 48 h thereafter to determine the expression of cervical COX-2, EP2, and EP4 mRNA by in situ hybridization, the concentration of hyaluronan by ELISA, and the proportion of smooth muscle and collagen by Masson's trichrome staining. Estradiol benzoate increased the mRNA expression of COX-2 and EP4 within 24 h after treatment (P < 0.05), whereas EP2 mRNA, hyaluronan, and the ratio of smooth muscle to collagen did not change within 48 h after treatment. The COX-2, EP2, and EP4 mRNA expression were greatest in the smooth muscle layers (P < 0.05) and least in the luminal epithelium (P < 0.05). In conclusion, we inferred that estradiol regulates cervical COX-2 and EP4 mRNA expression and may regulate cervical relaxation via the synthesis of prostaglandin E2 and activation of the PGE2 receptors EP2 and EP4.
© 2010 Elsevier Inc. All rights reserved.
Keyword Cervix
Cyclooxygenase-2
Estradiol
Extracellular matrix
Prostaglandin E receptors
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ
Additional Notes Available online 23 December 2009.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
School of Veterinary Science Publications
 
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