SAGE of the developing wheat caryopsis

McIntosh, Shane, Watson, Loraine, Bundock, Peter, Crawford, Allison, White, Jessica, Cordeiro, Giovanni, Barbary, Daniel, Rooke, Lee and Henry, Robert (2007) SAGE of the developing wheat caryopsis. Plant Biotechnology Journal, 5 1: 69-83. doi:10.1111/j.1467-7652.2006.00218.x

Author McIntosh, Shane
Watson, Loraine
Bundock, Peter
Crawford, Allison
White, Jessica
Cordeiro, Giovanni
Barbary, Daniel
Rooke, Lee
Henry, Robert
Title SAGE of the developing wheat caryopsis
Journal name Plant Biotechnology Journal   Check publisher's open access policy
ISSN 1467-7644
Publication date 2007-01-01
Sub-type Article (original research)
DOI 10.1111/j.1467-7652.2006.00218.x
Open Access Status DOI
Volume 5
Issue 1
Start page 69
End page 83
Total pages 15
Place of publication Oxford, England, U.K.
Publisher Wiley-Blackwell Publishing
Language eng
Formatted abstract
Understanding the development of the cereal caryopsis holds the future for metabolic engineering in the interests of enhancing global food production. We have developed a Serial Analysis of Gene Expression (SAGE) data platform to investigate the developing wheat (Triticum aestivum) caryopsis. LongSAGE libraries have been constructed at five time-points post-anthesis to coincide with key processes in caryopsis development. More than 90 000 LongSAGE tags have been sequenced generating 29 261 unique tag sequences across all five libraries. Tag abundance, generated from cumulative tag counts, provides insight into the redundancy and diversity of each library. Annotation of the 500 most abundant tags spanning development highlights the array of functional groups being expressed. The relative frequency of these more abundant transcripts allows quantitative analysis of patterns of expression during grain development. We have identified activities of cellular proliferation/differentiation, the accumulation of storage proteins and starch biosynthesis. The abundance of calcium-dependent protein kinases indicate their importance in signalling across development. Acquisition of a broad array of defence coincides with storage accumulation and is dominated by inhibitors of amylase activity. Differential expression profiles of abundant tags from each library reveal the coordinated expression of genes responsible for the cellular events constituting caryopsis development. This SAGE platform has also provided a resource of novel sequence and expression information including the identification of potentially useful promoter activities. Further investigations into both the abundant and low expressing transcripts will provide greater insight into wheat caryopsis development and assist in wheat improvement programmes. © 2006 Blackwell Publishing Ltd.
Keyword Abundant transcripts
Expression profiling
Hexaploid Wheat
Seed Development
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Alliance for Agriculture and Food Innovation
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Citation counts: TR Web of Science Citation Count  Cited 29 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 32 times in Scopus Article | Citations
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Created: Wed, 09 Feb 2011, 22:12:10 EST