Disposable amperometric immunosensor system for Rabbit IgG using a conducting polymer modified screen-printed electrode

Darain, Farzana, Park, Sang-Un and Shim, Yoon-Bo (2003). Disposable amperometric immunosensor system for Rabbit IgG using a conducting polymer modified screen-printed electrode. In: A. P. F. Turner, Selected papers from the Seventh World Congress on Biosensors. 7th World Congress on Biosensors, Kyoto, Japan, (773-780). 15-17 May 2002. doi:10.1016/S0956-5663(03)00004-6


Author Darain, Farzana
Park, Sang-Un
Shim, Yoon-Bo
Title of paper Disposable amperometric immunosensor system for Rabbit IgG using a conducting polymer modified screen-printed electrode
Conference name 7th World Congress on Biosensors
Conference location Kyoto, Japan
Conference dates 15-17 May 2002
Proceedings title Selected papers from the Seventh World Congress on Biosensors   Check publisher's open access policy
Journal name Biosensors and Bioelectronics   Check publisher's open access policy
Place of Publication Amsterdam, Netherlands
Publisher Elsevier BV
Publication Year 2003
Sub-type Fully published paper
DOI 10.1016/S0956-5663(03)00004-6
Open Access Status Not yet assessed
ISSN 0956-5663
1873-4235
Editor A. P. F. Turner
Volume 18
Issue 5-6
Start page 773
End page 780
Total pages 8
Language eng
Formatted Abstract/Summary
A disposable and mediatorless immunosensor based on a conducting polymer (5,2′:5′2″-terthiophene-3′-carboxylic acid) coated screen-printed carbon electrode has been developed using a separation-free homogeneous technique for the detection of rabbit IgG as a model analyte. Horseradish peroxidase (HRP) and streptavidin were covalently bonded with the polymer on the electrode and biotinylated antibody was immobilized on the electrode surface using avidin–biotin coupling. This sensor was based on the competitive assay between free and labeled antigen for the available binding sites of antibody. Glucose oxidase was used as a label and in the presence of glucose, H2O2 formed by the analyte–enzyme conjugate was reduced by the enzyme channeling via HRP bonded on the electrode. The catalytic current was monitored amperometrically at −0.35 V vs. Ag/AgCl and this method showed a linear range of RIgG concentrations from 0.5 to 2 μg/ml with standard deviation ±0.0145 (n=4). Detection limit was determined to be 0.33 μg/ml.
Keyword Electrochemical immunosensor
Conducting polymer
Screen-printed electrode
Glucose oxidase
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Conference Paper
Sub-type: Fully published paper
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
 
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Created: Tue, 08 Feb 2011, 02:26:50 EST by Dr Farzana Darain on behalf of Aust Institute for Bioengineering & Nanotechnology