On-chip detection of myoglobin based on fluorescence

Darain, Farzana, Yager, Paul, Gan, Kai Ling and Tjin, Swee Chuan (2009) On-chip detection of myoglobin based on fluorescence. Biosensors and Bioelectronics, 24 6: 1744-1755. doi:10.1016/j.bios.2008.09.004


Author Darain, Farzana
Yager, Paul
Gan, Kai Ling
Tjin, Swee Chuan
Title On-chip detection of myoglobin based on fluorescence
Journal name Biosensors and Bioelectronics   Check publisher's open access policy
ISSN 0956-5663
1873-4235
Publication date 2009-02-15
Sub-type Article (original research)
DOI 10.1016/j.bios.2008.09.004
Open Access Status Not Open Access
Volume 24
Issue 6
Start page 1744
End page 1755
Total pages 12
Place of publication Amsterdam, The Netherlands
Publisher Elsevier BV
Language eng
Formatted abstract
A disposable immunosensor cartridge was developed that allows antibodies to be immobilized on the surface for the detection of myoglobin, a marker for the early assessment of acute myocardial infarction (AMI) using fluorescence techniques. The anti-myoglobin antibody was immobilized on a polystyrene substrate based on covalent bonding via silanization. The immunosensor chip layers were fabricated from sheets by CO2-laser ablation. The functionalized polystyrene surfaces were characterized by contact angle measurement, X-ray photoelectron spectroscopy (XPS), and atomic force microscopy (AFM). After the antigen-antibody reaction as a sandwich enzyme-linked immunosorbent assay (ELISA) with a horseradish peroxidase-conjugated secondary antibody (HRP-anti-myoglobin), addition of fluorogenic substrate produced a fluorescent dye which was quantified on-chip using fluorescent technique. The immunosensor response was linear for myoglobin concentrations between 20 and 230 ng/ml (r = 0.991, n = 3). The detection limit was found to be 16 ng/ml, which is lower than the clinical cut-off value for myoglobin in healthy patients. This protocol could be extended to the detection of other important cardiac markers simultaneously in microchannels.
Keyword Cardiac marker
Fluorescence
Microfluidic card
Polystyrene
Surface modification
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
 
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Created: Tue, 08 Feb 2011, 01:49:04 EST by Dr Farzana Darain on behalf of Aust Institute for Bioengineering & Nanotechnology