Surface modification of permalloy (Ni80Fe20) nanoparticles for biomedical applications

Qin, Gaowu W., Darain, Farzana, Wang, Hui and Dimitrov, Krassen (2011) Surface modification of permalloy (Ni80Fe20) nanoparticles for biomedical applications. Journal of Nanoparticle Research, 13 1: 45-51. doi:10.1007/s11051-010-0101-5

Author Qin, Gaowu W.
Darain, Farzana
Wang, Hui
Dimitrov, Krassen
Title Surface modification of permalloy (Ni80Fe20) nanoparticles for biomedical applications
Formatted title
Surface modification of permalloy (Ni80Fe20) nanoparticles for biomedical applications
Journal name Journal of Nanoparticle Research   Check publisher's open access policy
ISSN 1388-0764
Publication date 2011-01-01
Year available 2010
Sub-type Article (original research)
DOI 10.1007/s11051-010-0101-5
Open Access Status Not yet assessed
Volume 13
Issue 1
Start page 45
End page 51
Total pages 7
Place of publication Dordrecht, The Netherlands
Publisher Springer Netherlands
Language eng
Formatted abstract
We report a simple and novel method for surface biofunctionalization onto recently reported Ni80Fe20 permalloy nanoparticles (~71 nm) and the immobilization of a model protein, IgG from human serum. The strategy of protein immobilization involved attachment of histidine-tagged streptavidin to the Ni80Fe20 nanoparticles via a non-covalent ligand binding followed by biotinylated human IgG binding on the nanoparticle surface using the specific high affinity avidin–biotin interaction. The biofunctionalization of Ni80Fe20 permalloy nanoparticles was confirmed by Fourier Transform InfraRed (FTIR) spectroscopy and protein denaturing gel electrophoresis (lithium dodecyl sulfate-polyacrylamide gel electrophoresis, LDS-PAGE). This protocol for surface functionalization of the novel nanometer-sized Ni80Fe20 permalloy particles with biological molecules could open diverse applications in disease diagnostics and drug delivery.
Keyword Ni80Fe20 permalloy
Magnetic nanoparticles
Protein immobilization
Avidin–biotin bridge
Gel electrophoresis
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online: 9 October 2010

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
Australian Institute for Bioengineering and Nanotechnology Publications
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Citation counts: TR Web of Science Citation Count  Cited 4 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 4 times in Scopus Article | Citations
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Created: Tue, 08 Feb 2011, 01:34:39 EST by Dr Farzana Darain on behalf of Aust Institute for Bioengineering & Nanotechnology