Flexible nanosomes (SECosomes) enable efficient siRNA delivery in cultured primary skin cells and in the viable epidermis of ex vivo human skin

Geusens, Barbara, Van Gele, Mireille, Braat, Sien, De Smedt, Stefaan C., Stuart, Marc C. A., Prow, Tarl W., Sanchez, Washington, Roberts, Michael S., Sanders, Niek N. and Lambert, Jo (2010) Flexible nanosomes (SECosomes) enable efficient siRNA delivery in cultured primary skin cells and in the viable epidermis of ex vivo human skin. Advanced Functional Materials, 20 23: 4077-4090. doi:10.1002/adfm.201000484


Author Geusens, Barbara
Van Gele, Mireille
Braat, Sien
De Smedt, Stefaan C.
Stuart, Marc C. A.
Prow, Tarl W.
Sanchez, Washington
Roberts, Michael S.
Sanders, Niek N.
Lambert, Jo
Title Flexible nanosomes (SECosomes) enable efficient siRNA delivery in cultured primary skin cells and in the viable epidermis of ex vivo human skin
Journal name Advanced Functional Materials   Check publisher's open access policy
ISSN 1616-301X
1616-3028
Publication date 2010-12-08
Year available 2017
Sub-type Article (original research)
DOI 10.1002/adfm.201000484
Open Access Status Not yet assessed
Volume 20
Issue 23
Start page 4077
End page 4090
Total pages 14
Place of publication Weinheim, Germany
Publisher Wiley - V C H Verlag
Language eng
Subject 2504 Electronic, Optical and Magnetic Materials
2502 Biomaterials
3104 Condensed Matter Physics
1603 Electrochemistry
Abstract The extent to which nanoscale-engineered systems cross intact human skin and can exert pharmacological effects in viable epidermis is controversial. This research seeks to develop a new lipid-based nanosome that enables the effective delivery of siRNA into human skin. The major finding is that an ultraflexible siRNA-containing nanosome-prepared using DOTAP, cholesterol, sodium cholate, and 30% ethanol-penetrates into the epidermis of freshly excised intact human skin and is able to enter into the keratinocytes. The nanosomes, called surfactant-ethanol-cholesterol-osomes (SECosomes), show excellent size, surface charge, morphology, deformability, transfection efficiency, stability, and skin penetration capacity after complexation with siRNA. Importantly, these nanosomes have ideal characteristics for siRNA encapsulation, in that the siRNA is stable for at least 4 weeks, they enable highly efficient transfection of in vitro cultured cells, and are shown to transport siRNA delivery through intact human skin where changes in the keratinocyte cell state are demonstrated. It is concluded that increasing flexibility in nanosomes greatly enhances their ability to cross the intact human epidermal membrane and to unload their payload into targeted epidermal cells. © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Keyword FLIM
Liposomes
Multiphoton tomography
Topical siRNA delivery
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID APP1062935
APP1099021
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2011 Collection
School of Medicine Publications
 
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Created: Sun, 30 Jan 2011, 10:07:59 EST