Escherichia coli O25b-ST131: a pandemic, multiresistant, community-associated strain

Rogers, BA, Sidjabat, HE and Paterson, DL (2011) Escherichia coli O25b-ST131: a pandemic, multiresistant, community-associated strain. Journal of Antimicrobial Chemotherapy, 66 1: 1-14. doi:10.1093/jac/dkq415


Author Rogers, BA
Sidjabat, HE
Paterson, DL
Title Escherichia coli O25b-ST131: a pandemic, multiresistant, community-associated strain
Formatted title
Escherichia coli O25b-ST131: a pandemic, multiresistant, community-associated strain
Journal name Journal of Antimicrobial Chemotherapy   Check publisher's open access policy
ISSN 0305-7453
Publication date 2011-01-01
Year available 2011
Sub-type Article (original research)
DOI 10.1093/jac/dkq415
Open Access Status DOI
Volume 66
Issue 1
Start page 1
End page 14
Total pages 14
Place of publication Oxford, England, U.K.
Publisher Oxford University Press
Language eng
Subject 3004 Pharmacology
2736 Pharmacology (medical)
2725 Infectious Diseases
Abstract Uropathogenic Escherichia coli (UPEC) is the cause of ~75% of all urinary tract infections (UTIs) and is increasingly associated with multidrug resistance. This includes UPEC strains from the recently emerged and globally disseminated sequence type 131 (ST131), which is now the dominant fluoroquinolone-resistant UPEC clone worldwide. Most ST131 strains are motile and produce H4-type flagella. Here, we applied a combination of saturated Tn5 mutagenesis and transposon directed insertion site sequencing (TraDIS) as a high throughput genetic screen and identified 30 genes associated with enhanced motility of the reference ST131 strain EC958. This included 12 genes that repress motility of E. coli K-12, four of which (lrhA, ihfA, ydiV, lrp) were confirmed in EC958. Other genes represented novel factors that impact motility, and we focused our investigation on characterisation of the mprA, hemK and yjeA genes. Mutation of each of these genes in EC958 led to increased transcription of flagellar genes (flhD and fliC), increased expression of the FliC flagellin, enhanced flagella synthesis and a hyper-motile phenotype. Complementation restored all of these properties to wild-type level. We also identified Tn5 insertions in several intergenic regions (IGRs) on the EC958 chromosome that were associated with enhanced motility; this included flhDC and EC958_1546. In both of these cases, the Tn5 insertions were associated with increased transcription of the downstream gene(s), which resulted in enhanced motility. The EC958_1546 gene encodes a phage protein with similarity to esterase/deacetylase enzymes involved in the hydrolysis of sialic acid derivatives found in human mucus. We showed that over-expression of EC958_1546 led to enhanced motility of EC958 as well as the UPEC strains CFT073 and UTI89, demonstrating its activity affects the motility of different UPEC strains. Overall, this study has identified and characterised a number of novel factors associated with enhanced UPEC motility.
Keyword Infectious Diseases
Microbiology
Pharmacology & Pharmacy
Infectious Diseases
Microbiology
Pharmacology & Pharmacy
INFECTIOUS DISEASES
MICROBIOLOGY
PHARMACOLOGY & PHARMACY
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2012 Collection
School of Medicine Publications
 
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Created: Sun, 30 Jan 2011, 10:04:15 EST