Novel trypanosome Trypanosoma gilletti sp. (Euglenozoa: Trypanosomatidae) and the extension of the host range of Trypanosoma copemani to include the koala (Phascolarctos cinereus)

McInnes, L. M., Hanger, J., Simmons, G., Reid, S. A. and Ryan, U. M. (2011) Novel trypanosome Trypanosoma gilletti sp. (Euglenozoa: Trypanosomatidae) and the extension of the host range of Trypanosoma copemani to include the koala (Phascolarctos cinereus). Parasitology, 138 1: 59-70. doi:10.1017/S0031182010000971


Author McInnes, L. M.
Hanger, J.
Simmons, G.
Reid, S. A.
Ryan, U. M.
Title Novel trypanosome Trypanosoma gilletti sp. (Euglenozoa: Trypanosomatidae) and the extension of the host range of Trypanosoma copemani to include the koala (Phascolarctos cinereus)
Formatted title
Novel trypanosome Trypanosoma gilletti sp. (Euglenozoa: Trypanosomatidae) and the extension of the host range of Trypanosoma copemani to include the koala (Phascolarctos cinereus)
Journal name Parasitology   Check publisher's open access policy
ISSN 0031-1820
1469-8161
Publication date 2011-01-01
Year available 2010
Sub-type Article (original research)
DOI 10.1017/S0031182010000971
Open Access Status DOI
Volume 138
Issue 1
Start page 59
End page 70
Total pages 12
Place of publication Cambridge, United Kingdom
Publisher Cambridge University Press
Language eng
Abstract Infection of C57Bl/6 mice by pleomorphic African trypanosomes Trypanosoma brucei and T. congolense is characterized by parasitemia waves coupled with the production of systemic levels of TNF. This cytokine is known to control T. brucei growth, but also to contribute to tissue damage, shortening the survival time of infected mice. Using a dominant-negative version of TNF to discriminate between the effects of the membrane-form versus the soluble form of TNF, we show that the second form is involved in neither parasite control nor induction of liver injury. Therefore, soluble TNF is likely not a major contributor to disease outcome. We propose that membrane-bound TNF is responsible for both T. brucei control and host pathology.
Formatted abstract
Trypanosoma irwini was previously described from koalas and we now report the finding of a second novel species, T. gilletti, as well as the extension of the host range of Trypanosoma copemani to include koalas. Phylogenetic analysis at the 18S rDNA and gGAPDH loci demonstrated that T. gilletti was genetically distinct with a genetic distance (±s.e.) at the 18S rDNA locus of 2.7±0.5% from T. copemani (wombat). At the gGAPDH locus, the genetic distance (±s.e.) of T. gilletti was 8.7±1.1% from T. copemani (wombat). Trypanosoma gilletti was detected using a nested trypanosome 18S rDNA PCR in 3/139 (∼2%) blood samples and in 2/29 (∼7%) spleen tissue samples from koalas whilst T. irwini was detected in 72/139 (∼52%) blood samples and T. copemani in 4/139 (∼3%) blood samples from koalas. In addition, naturally occurring mixed infections were noted in 2/139 (∼1.5%) of the koalas tested.
© 2010 Cambridge University Press.
Keyword Trypanosoma spp.
Trypanosoma gilletti
Trypanosoma copemani
Trypanosoma irwini
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID 669007
Institutional Status UQ
Additional Notes First published online 21 July 2010.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Veterinary Science Publications
 
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Created: Sun, 23 Jan 2011, 10:01:01 EST