In vivo and in vitro starch digestion: Are current in vitro techniques adequate?

Hasjim, Jovin, Lavau, Gautier Cesbron, Gidley, Michael J. and Gilbert, Robert G. (2010) In vivo and in vitro starch digestion: Are current in vitro techniques adequate?. Biomacromolecules, 11 12: 3600-3608. doi:10.1021/bm101053y

Author Hasjim, Jovin
Lavau, Gautier Cesbron
Gidley, Michael J.
Gilbert, Robert G.
Title In vivo and in vitro starch digestion: Are current in vitro techniques adequate?
Journal name Biomacromolecules   Check publisher's open access policy
ISSN 1525-7797
Publication date 2010-12-13
Sub-type Article (original research)
DOI 10.1021/bm101053y
Volume 11
Issue 12
Start page 3600
End page 3608
Total pages 9
Place of publication Washington, DC, United States
Publisher American Chemical Society
Language eng
Formatted abstract
The time evolution of the size distributions of (fully branched and debranched) starch molecules during in vivo and in vitro digestion was analyzed using size exclusion chromatography (SEC) and compared. In vivo digesta were collected from the small intestine of pigs fed with raw normal maize starch; in vitro digestion was carried out on the same diet fed to the pigs using a method simulating digestion in the mouth, stomach, and small intestine. A qualitative difference was observed between the in vitro and the in vivo digestion. The former showed a degradation of starch molecules to a more uniform size, whereas the in vivo digestion preserved the size distribution of native starch before producing a multimodal distribution, the heterogeneous nature of which current in vitro methods do not reproduce. The use of in vitro digestion to infer in vivo digestion patterns and, hence, potential nutrition benefits need to take account of this phenomenon.
© 2010 American Chemical Society.
Keyword In vitro digestion
In vivo digestion
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
Centre for Nutrition and Food Sciences Publications
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Citation counts: TR Web of Science Citation Count  Cited 59 times in Thomson Reuters Web of Science Article | Citations
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Created: Wed, 05 Jan 2011, 22:03:02 EST by Mr Jovin Hasjim on behalf of Centre for Nutrition and Food Sciences