High-performance liquid chromatographic determination of amoxicillin in urine using solid-phase, ion-pair extraction and ultraviolet detection

Chulavatnatol, Suvatna and Charles, Bruce G. (1993) High-performance liquid chromatographic determination of amoxicillin in urine using solid-phase, ion-pair extraction and ultraviolet detection. Journal of Chromatography B, 615 1: 91-96. doi:10.1016/0378-4347(93)80294-E


Author Chulavatnatol, Suvatna
Charles, Bruce G.
Title High-performance liquid chromatographic determination of amoxicillin in urine using solid-phase, ion-pair extraction and ultraviolet detection
Journal name Journal of Chromatography B
ISSN 0378-4347
1873-376X
Publication date 1993-05-01
Sub-type Article (original research)
DOI 10.1016/0378-4347(93)80294-E
Volume 615
Issue 1
Start page 91
End page 96
Total pages 6
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Formatted abstract
A simple, robust high-performanceliquidchromatographic method is described for assaying amoxicillin in urine. Sample clean-up involved solid-phase, ion-pairextraction onto Sep-Pak C18 cartridges followed by elution with acetonitrile (3%, v/v) in pH 4.85 buffer. Separations were performed on an Ultrasphere C18 column with a mobile phase comprising acetonitrile (32.5 ml) and pH 7.1 phosphate buffer (0.01 M, 1000 ml). Peaks were detected at 229 nm. Recovery was greater than 94%. Plots of peak area against urinary amoxicillin concentration were linear (r > 0.999) from 5 to 500 mg/l. Between-day and within-day imprecision (coefficient of variation) ranged between 3.0 and 15.5%. Inaccuracy was 7.1%, or less.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Pharmacy Publications
 
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Created: Mon, 20 Dec 2010, 23:51:44 EST