A new method for assaying propantheline and its degradation product, xanthene-9-carboxylic acid using high-performance liquid chromatography

Charles, B.G. and Ravenscroft, P.J. (1983) A new method for assaying propantheline and its degradation product, xanthene-9-carboxylic acid using high-performance liquid chromatography. Journal of Pharmaceutical Sciences, 72 1: 96-98. doi:10.1002/jps.2600720126


Author Charles, B.G.
Ravenscroft, P.J.
Title A new method for assaying propantheline and its degradation product, xanthene-9-carboxylic acid using high-performance liquid chromatography
Journal name Journal of Pharmaceutical Sciences   Check publisher's open access policy
ISSN 0022-3549
1520-6017
Publication date 1983-01-01
Year available 1983
Sub-type Other
DOI 10.1002/jps.2600720126
Volume 72
Issue 1
Start page 96
End page 98
Total pages 3
Place of publication Hoboken, NJ, United States
Publisher John Wiley & Sons
Language eng
Abstract A rapid, specific, and precise high-performance liquid chromatographic method is described for the simultaneous analysis of propantheline bromide and its hydrolysis product, xanthene-9-carboxylic acid. Reversed-phase chromatography was conducted using a mobile phase of 40:60, acetonitrile-0.05 M phosphate buffer (pH 2.5) delivered at 2 ml/min. Detection was at 254 nm. Methantheline bromide (internal standard), propantheline bromide, and xanthene-9-carboxylic acid gave retention times of 4.1, 5.4, and 8.3 min, respectively. Within-day, between-day, and total precision (CV) for assay of 15 mg/10 ml propantheline bromide are 1.2, 1.1, and 1.6%, respectively (n = 20). Similar precision was obtained for xanthene-9-carboxylic acid. The limit of detection was 2 ng. The assay is useful for routine quality assurance of propantheline in dosage forms and for stability and kinetic studies.
Keyword Propantheline—new method of high-performance liquid chromatographic assay, degradation product, xanthene-9-carboxylic acid
Xanthene-9-carboxylic acid—degradation product, new method of high-performance liquid chromatographic assay of propantheline
High-performance liquid chromatography—new method for assaying propantheline and its degradation product, xanthene-9-carboxylic acid
Q-Index Code CX
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Note

Document type: Journal Article
Sub-type: Other
Collection: School of Pharmacy Publications
 
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Created: Mon, 20 Dec 2010, 23:49:27 EST