Evidence for bacteriophage activity causing community and performance changes in a phosphorus-removal activated sludge

Barr, Jeremy J., Slater, Frances R., Fukushima, Toshikazu and Bond, Philip L. (2010) Evidence for bacteriophage activity causing community and performance changes in a phosphorus-removal activated sludge. FEMS Microbiology Ecology, 74 3: 631-642. doi:10.1111/j.1574-6941.2010.00967.x


Author Barr, Jeremy J.
Slater, Frances R.
Fukushima, Toshikazu
Bond, Philip L.
Title Evidence for bacteriophage activity causing community and performance changes in a phosphorus-removal activated sludge
Journal name FEMS Microbiology Ecology   Check publisher's open access policy
ISSN 0168-6496
1574-6941
Publication date 2010-12-01
Sub-type Article (original research)
DOI 10.1111/j.1574-6941.2010.00967.x
Volume 74
Issue 3
Start page 631
End page 642
Total pages 12
Editor Max Häggblom
Place of publication Oxford, U.K.
Publisher Wiley-Blackwell Publishing
Collection year 2011
Language eng
Formatted abstract
Bacteria are known to play important roles in biogeochemical cycles and biotechnology processes, but little is known about the influence of bacteriophage on these processes. A major impediment to the study of host–bacteriophage interactions is that the bacteria and their bacteriophage are often not available in a pure culture. In this study, we detected an unexpected decline in the phosphorus-removal performance of a granular laboratory-scale wastewater treatment reactor. Investigations by FISH, transmission electron microscopy and proteomics led us to hypothesize that a bacteriophage infection of the uncultured Candidatus‘Accumulibacter phosphatis’ was responsible for the decline in performance. Further experiments demonstrated that the addition of a putative bacteriophage-rich supernatant, obtained from the previous failed reactor to phosphorus-removal reactors, caused a decrease in the abundance of Accumulibacter in both granular and floccular activated sludges. This coincided with increases in bacteriophage-like particles and declining phosphorus-removal performance. The granular sludge did not recover after the attack, but the floccular sludge regained Accumulibacter numbers and phosphorus-removal performance. These findings suggest that bacteriophage may play a significant role in determining the structure and function of bacterial communities in activated sludges.
Keyword Accumulibacter
Activated sludge
Bacteriophage
EBPR
Phosphorus removal
Waste-water treatment
Glycogen-accumulating organisms
Aerobic granular sludge
Targeted oligonucleotide probes
Provides acquired-resistance
Sequencing batch reactor
Phage-host system
SYBR green-I
Of-the-art
Microbial-populations
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
Advanced Water Management Centre Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 21 times in Thomson Reuters Web of Science Article | Citations
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Created: Sun, 12 Dec 2010, 10:08:23 EST