Direct activation of human polymorphonuclear leukocytes by Pseudomonas aeruginosa

Mai, G. T., Seow, W. K., McCormack, J. G. and Thong, Y. H. (1989) Direct activation of human polymorphonuclear leukocytes by Pseudomonas aeruginosa. International Archives of Allergy and Applied Immunology, 90 4: 358-363. doi:10.1159/000235053

Author Mai, G. T.
Seow, W. K.
McCormack, J. G.
Thong, Y. H.
Title Direct activation of human polymorphonuclear leukocytes by Pseudomonas aeruginosa
Journal name International Archives of Allergy and Applied Immunology   Check publisher's open access policy
ISSN 0020-5915
Publication date 1989-12-01
Year available 1989
Sub-type Article (original research)
DOI 10.1159/000235053
Open Access Status Not yet assessed
Volume 90
Issue 4
Start page 358
End page 363
Total pages 6
Place of publication BASEL
Publisher KARGER
Language eng
Subject 2723 Immunology and Allergy
2403 Immunology
Abstract Brief exposure of polymorphonuclear leukocytes (PMNs) to unopsonized Pseudomonas aeruginosa resulted in significant enhancement of PMN adherence and 3H-deoxyglucose uptake, two important indicators of PMN activation. Dose-response and time-course experiments show that maximal stimulation of PMNs occurred at a PMN: Bacteria ratio of 10:1 and exposure time of 10 min. The PMN-stimulatory capacity of P. aeruginosa was completely or partially abolished by treatment with formalin, heat, ultrasonication and ultraviolet (UV) irradiation, all of which affect the integrity of ligands on the bacteria surface. The putative glycoprotein ligand(s) contains galactose rather than glucose as shown by the ability of galactose in the medium to abrogate PMN-stimulatory activity. This PMN-stimulatory activity was not mediated by soluble factor(s) released from either P. aeruginosa or PMNs as shown by experiments with appropriate supernatants. Finally, PMN-stimulating activity was also observed with P. mattophilia and P. pseudomallei, but not by the other Pseudomonas spp. such as P. cepacia and P. fluorescens. These results suggest that P. aeruginosa and other Pseudomonas spp. may have little capacity to circumvent PMN host defences. This may account in part for their limited pathogenic role as opportunistic micro-organisms, for the severe inflammatory lesions seen in Pseudomonas infections when they do occur, and is consistent with the fact that infection with these organisms is commonly associated with impaired quantity or function of PMNs.
Keyword Allergy
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Dentistry Publications
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Created: Thu, 18 Nov 2010, 21:01:42 EST