Identification of a mutation in the para-sodium channel gene of the cattle tick Rhipicephalus microplus associated with resistance to flumethrin but not to cypermethrin

Jonsson, N. N., Cutulle, C., Corley, S. W. and Seddon, J. M. (2010) Identification of a mutation in the para-sodium channel gene of the cattle tick Rhipicephalus microplus associated with resistance to flumethrin but not to cypermethrin. International Journal for Parasitology, 40 14: 1659-1664. doi:10.1016/j.ijpara.2010.07.006


Author Jonsson, N. N.
Cutulle, C.
Corley, S. W.
Seddon, J. M.
Title Identification of a mutation in the para-sodium channel gene of the cattle tick Rhipicephalus microplus associated with resistance to flumethrin but not to cypermethrin
Formatted title
Identification of a mutation in the para-sodium channel gene of the cattle tick Rhipicephalus microplus associated with resistance to flumethrin but not to cypermethrin
Journal name International Journal for Parasitology   Check publisher's open access policy
ISSN 0020-7519
1879-0135
Publication date 2010-12-01
Sub-type Article (original research)
DOI 10.1016/j.ijpara.2010.07.006
Volume 40
Issue 14
Start page 1659
End page 1664
Total pages 6
Place of publication Oxford, United Kingdom
Publisher Elsevier Science
Collection year 2011
Language eng
Abstract A mutation in the domain II S4-5 linker region of the para-sodium channel gene has been associated previously with synthetic pyrethroid (SP) resistance in the cattle tick (Rhipicephalus microplus) in Australia. This is a C → A mutation at nucleotide position 190, which results in a leucine to isoleucine amino acid substitution (L64. I). In a survey of 15 cattle tick populations with known SP resistance status, sourced from Queensland and New South Wales in Australia, there was a strong relationship (r=0.98) between the proportion of ticks carrying the L64. I homozygous resistant genotype and the survival percentage after exposure to a discriminating concentration of cypermethrin in the bioassay, as expected. However, among populations resistant only to flumethrin, the L64. I homozygous genotype was not found. The sequence obtained for a 167. bp region including domain II S4-5 linker in flumethrin-resistant ticks identified a G → T non-synonymous mutation at nucleotide position 214 that results in a glycine to valine substitution (G72. V). The frequency of the G72. V homozygous genotype in each population was found to be moderately related to the survival percentage at the discriminating concentration of flumethrin in the larval packet test (r=0.74). However, a much stronger relationship between genotype and resistance to flumethrin was observed when the heterozygotes of L64. I and G72. V were added to the G72V homozygotes (r=0.93). These results suggest that there is an interaction between the two mutations in the same gene, such that flumethrin resistance might be conferred by either two copies of the G72. V mutation or by being a L64. I and G72. V heterozygote. © Australian Society for Parasitology Inc.
Formatted abstract
A mutation in the domain II S4–5 linker region of the para-sodium channel gene has been associated previously with synthetic pyrethroid (SP) resistance in the cattle tick (Rhipicephalus microplus) in Australia. This is a C → A mutation at nucleotide position 190, which results in a leucine to isoleucine amino acid substitution (L64I). In a survey of 15 cattle tick populations with known SP resistance status, sourced from Queensland and New South Wales in Australia, there was a strong relationship (r = 0.98) between the proportion of ticks carrying the L64I homozygous resistant genotype and the survival percentage after exposure to a discriminating concentration of cypermethrin in the bioassay, as expected. However, among populations resistant only to flumethrin, the L64I homozygous genotype was not found. The sequence obtained for a 167 bp region including domain II S4–5 linker in flumethrin-resistant ticks identified a G → T non-synonymous mutation at nucleotide position 214 that results in a glycine to valine substitution (G72V). The frequency of the G72V homozygous genotype in each population was found to be moderately related to the survival percentage at the discriminating concentration of flumethrin in the larval packet test (r = 0.74). However, a much stronger relationship between genotype and resistance to flumethrin was observed when the heterozygotes of L64I and G72V were added to the G72V homozygotes (r = 0.93). These results suggest that there is an interaction between the two mutations in the same gene, such that flumethrin resistance might be conferred by either two copies of the G72V mutation or by being a L64I and G72V heterozygote.
© 2010 Australian Society for Parasitology Inc.

Keyword Acaricide resistance
Cattle tick
Rhipicephalus (Boophilus) microplus
Synthetic pyrethroid
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Veterinary Science Publications
 
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Created: Wed, 17 Nov 2010, 20:24:47 EST by AProf Jennifer Seddon on behalf of School of Veterinary Science