Protein tyrosine kinase 2 beta (PTK2B), but not focal adhesion kinase (FAK), is expressed in a sexually dimorphic pattern in developing mouse gonads

Beverdam, Annemiek, Svingen, Terje, Bagheri-Fam, Stefan, McClive, Peter, Sinclair, Andrew H., Harley, Vincent R. and Koopman, Peter (2010) Protein tyrosine kinase 2 beta (PTK2B), but not focal adhesion kinase (FAK), is expressed in a sexually dimorphic pattern in developing mouse gonads. Developmental Dynamics, 239 10: 2735-2741. doi:10.1002/dvdy.22396


Author Beverdam, Annemiek
Svingen, Terje
Bagheri-Fam, Stefan
McClive, Peter
Sinclair, Andrew H.
Harley, Vincent R.
Koopman, Peter
Title Protein tyrosine kinase 2 beta (PTK2B), but not focal adhesion kinase (FAK), is expressed in a sexually dimorphic pattern in developing mouse gonads
Journal name Developmental Dynamics   Check publisher's open access policy
ISSN 1058-8388
Publication date 2010-10-01
Year available 2010
Sub-type Article (original research)
DOI 10.1002/dvdy.22396
Open Access Status Not yet assessed
Volume 239
Issue 10
Start page 2735
End page 2741
Total pages 7
Place of publication Hoboken, NJ, U.S.A
Publisher John Wiley & Sons
Language eng
Abstract Sexual reproduction is essential for the propagation and the maintenance of fitness of our species, and is dependent on the correct development of the bipotential genital ridges into testes and ovaries. Although several transcription factors, secreted signaling molecules, and their receptors have been found to be important for testis determination and early gonad development, comparatively little research has been carried out into intracellular signal transduction pathways activated during these processes. Focal adhesion kinase (FAK) and protein tyrosine kinase 2 beta (PTK2B) form one group of cytosolic tyrosine kinases that are known to be important for processes such as cell proliferation, differentiation, and motility. Here, we describe the temporal and spatial expression patterns of Fak and Ptk2b mRNA and protein during sex determination and early gonadogenesis in mouse embryos. Ptk2b mRNA and PTK2B protein were expressed in testes from 11.5 days post coitum onward, predominantly in developing Sertoli cells, in a SOX9-dependent manner. Fak mRNA and FAK protein were expressed in gonads of both sexes at all stages examined. Our data suggest cell type- and stage-specific roles for PTK2B during early testis development. Developmental Dynamics 239:2735–2741, 2010. © 2010 Wiley-Liss, Inc.
Keyword Fak
Ptk2b
gonad
sex determination
Sertoli cells
Sex Reversal
Testis Differentiation
Signaling events
In-vitro
Es Cells
Mice
PYK2
Phosphorylation
SRC
Activation
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID 334314
Institutional Status UQ
Additional Notes Article first published online: 24 AUG 2010

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
Institute for Molecular Bioscience - Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 3 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 3 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Sun, 07 Nov 2010, 10:14:20 EST