Detection of Polymerase Chain Reaction Fragments Using a Conducting Polymer-Modified Screen Printed Electrode in a Microfluidic Device

Shiddiky, Muhammad J. A., Park , Deog-Su and Shim, Yoon-Bo (2005) Detection of Polymerase Chain Reaction Fragments Using a Conducting Polymer-Modified Screen Printed Electrode in a Microfluidic Device. Electrophoresis, 26 24: 4656-4663. doi:10.1002/elps.200500447


Author Shiddiky, Muhammad J. A.
Park , Deog-Su
Shim, Yoon-Bo
Title Detection of Polymerase Chain Reaction Fragments Using a Conducting Polymer-Modified Screen Printed Electrode in a Microfluidic Device
Journal name Electrophoresis   Check publisher's open access policy
ISSN 0173-0835
Publication date 2005-11-11
Sub-type Article (original research)
DOI 10.1002/elps.200500447
Open Access Status
Volume 26
Issue 24
Start page 4656
End page 4663
Total pages 8
Editor Ziad J. El Rassi
Place of publication Germany
Publisher Wiley - V C H Verlag GmbH & Co
Language eng
Subject C1
Abstract A simple and fast method for electrochemical detection of amplified fragments by PCR was successfully developed using CE in a microfluidic device with a modified screenprinted carbon electrode (SPCE). The surfaces of the SPCE were modified with poly- 5,2’-5’,2’’-terthiophene-3’-carboxylic acid, which improves the analysis performance by lowering the detection potential, enhancing the S/N characteristics, and avoiding electrode poisoning. DNA fragments amplified by PCR were separated within 210 s in a 75.5 mm-long coated-separation channel at a separation field strength of 2200 V/cm. To minimize the sample adsorption into the inner surface of the capillary wall, which disturbs the separation, a dynamically coated capillary with an acrylamide solution was used. Furthermore, the analysis procedure was simplified and rendered reproducible by using 0.50% w/v hydroxyethylcellulose as a separation matrix in a coated channel. The reproducibility of the analysis employing the coated channel yielded RSD of 4.3% for the peak areas and 1.4% for the migration times in eight repetitive measurements at a modified electrode, compared with 21.3 and 9.4% for a bare electrode. The sensitivity of the assay was 18.74 pAs/(pg/mL) with a detection limit of 584.31 6 1.3 fg/mL.
Keyword Conducting polymer
Electrochemical detection
Microfluidic device
Miniaturization
PCR
Screen-printed electrode
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
 
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Created: Tue, 02 Nov 2010, 21:30:52 EST by Dr Muhammad J. A. Shiddiky on behalf of Aust Institute for Bioengineering & Nanotechnology