Simultaneous analysis of nitrate and nitrite in a microfluidic device with a Cu-complex-modified electrode

Shiddiky, Muhammad J. A., Won, Mi-Sook and Shim, Yoon-Bo (2006) Simultaneous analysis of nitrate and nitrite in a microfluidic device with a Cu-complex-modified electrode. Electrophoresis, 27 22: 4545-4554. doi:10.1002/elps.200600240


Author Shiddiky, Muhammad J. A.
Won, Mi-Sook
Shim, Yoon-Bo
Title Simultaneous analysis of nitrate and nitrite in a microfluidic device with a Cu-complex-modified electrode
Journal name Electrophoresis   Check publisher's open access policy
ISSN 0173-0835
1522-2683
Publication date 2006-10-23
Sub-type Article (original research)
DOI 10.1002/elps.200600240
Volume 27
Issue 22
Start page 4545
End page 4554
Total pages 10
Editor Ziad J. El Rassi
Place of publication Germany
Publisher Wiley - V C H Verlag GmbH & Co
Language eng
Subject C1
Abstract A CE microsystem coupled with a microchip and a copper–(3-mercaptopropyl) trimethoxysilane (Cu–MPS) complex-modified carbon paste electrode (CPE) was developed for the simultaneous analysis of nitrite and nitrate. The method is based on the electrocatalytic reduction of both analytes with the modified electrode. The Cu–MPS complex was characterized by voltammetric, XPS, and FT-IR analyses. Experimental parameters affecting the sensitivity of the modified electrode were assessed and optimized. The best separation was achieved in a 60 mm separation channel filled with a 20 mM acetate buffer of pH 5.0 containing 3.0 mM CTAB at separation field strength of 2250 V/cm within 90 s. The detection potential for the simultaneous analysis of nitrite and nitrate was found to be 2225 mV versus Ag/AgCl. A reproducible response (RSD of 3.2% (nitrite) and 2.8% (nitrate), n = 8) for repetitive sample injections reflected the negligible electrode fouling at the modified CPE. The interference effect was examined for other inorganic ions and biological compounds. A wide hydrodynamic range between 0.25 and 120 mM was observed for analyzing nitrite and nitrate with the sensitivities of 0.069 6 0.003 and 0.065 6 0.002 nA/mM, and the detection limits, based on S/N = 3, were found to be 0.09 6 0.007 and 0.08 6 0.009 mM, respectively. The applicability of the method to water and urine samples analyses was demonstrated.
Keyword Cu-complex-modified electrode
Electrochemical detection
Microfluidic device
Microchip CE
Nitrite and nitrate
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
 
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Created: Tue, 02 Nov 2010, 21:20:13 EST by Dr Muhammad J. A. Shiddiky on behalf of Aust Institute for Bioengineering & Nanotechnology