Enzyme activity in the aestivating Green-striped burrowing frog (Cyclorana alboguttata)

Mantle, Beth L., Guderley, Helga, Hudson, Nicholas J. and Franklin, Craig E. (2010) Enzyme activity in the aestivating Green-striped burrowing frog (Cyclorana alboguttata). Journal of Comparative Physiology B-biochemical Systemic And Environmental Physiology, 180 7: 1033-1043. doi:10.1007/s00360-010-0471-0


Author Mantle, Beth L.
Guderley, Helga
Hudson, Nicholas J.
Franklin, Craig E.
Title Enzyme activity in the aestivating Green-striped burrowing frog (Cyclorana alboguttata)
Formatted title
Enzyme activity in the aestivating Green-striped burrowing frog (Cyclorana alboguttata)
Journal name Journal of Comparative Physiology B-biochemical Systemic And Environmental Physiology   Check publisher's open access policy
ISSN 0174-1578
1432-136X
Publication date 2010-10-01
Sub-type Article (original research)
DOI 10.1007/s00360-010-0471-0
Volume 180
Issue 7
Start page 1033
End page 1043
Total pages 11
Place of publication Berlin, Germany
Publisher Springer International
Language eng
Abstract Green-striped burrowing frogs (Cyclorana alboguttata) can depress their resting metabolism by more than 80% during aestivation. Previous studies have shown that this species is able to withstand long periods of immobilisation during aestivation while apparently maintaining whole muscle mass and contractile performance. The aim of this study was to determine the effect of prolonged aestivation on the levels of metabolic enzymes (CCO, LDH and CS) in functionally distinct skeletal muscles (cruralis, gastrocnemius, sartorius, iliofibularis and rectus abdominus) and liver of C. alboguttata. CS activity was significantly reduced in all tissues except for the cruralis, gastrocnemius and the liver. LDH activity was significantly reduced in the sartorius and rectus abdominus, but remained at control (active) levels in the other tissues. CCO activity was significantly reduced in the gastrocnemius and rectus abdominus, and unchanged in the remaining tissues. Muscle protein was significantly reduced in the sartorius and iliofibularis during aestivation, and unchanged in the remaining muscles. The results suggest that the energy pathways involved in the production and consumption of ATP are remodelled during prolonged aestivation but selective. Remodelling and subsequent down-regulation of metabolic activity seem to target the smaller non-jumping muscles, while the jumping muscles retain enzyme activities at control levels during aestivation. These results suggest a mechanism by which aestivating C. alboguttata are able to maintain metabolic depression while ensuring that the functional capacity of critical muscles is not compromised upon emergence from aestivation. © 2010 Springer-Verlag
Keyword Aestivation
Metabolism
Enzyme
Skeletal muscle
Cyclorana alboguttata
Atrophy
Metabolic-rate depression
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Received: 29 April 2009 / Revised: 11 March 2010 / Accepted: 13 March 2010 / Published online: 3 April 2010

 
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Created: Sun, 24 Oct 2010, 10:11:39 EST