Targeted, needle-free vaccinations in skin using multi layered, densely-packed dissolving microprojection arrays

Raphael, Anthony P., Prow, Tarl W., Crichton, Michael L., Chen, Xianfeng, Fernando, Germain J. P. and Kendall, Mark A. F. (2010) Targeted, needle-free vaccinations in skin using multi layered, densely-packed dissolving microprojection arrays. Small, 6 16: 1785-1793. doi:10.1002/smll.201000326

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Author Raphael, Anthony P.
Prow, Tarl W.
Crichton, Michael L.
Chen, Xianfeng
Fernando, Germain J. P.
Kendall, Mark A. F.
Title Targeted, needle-free vaccinations in skin using multi layered, densely-packed dissolving microprojection arrays
Journal name Small   Check publisher's open access policy
ISSN 1613-6810
Publication date 2010-08-01
Year available 2010
Sub-type Article (original research)
DOI 10.1002/smll.201000326
Open Access Status Not yet assessed
Volume 6
Issue 16
Start page 1785
End page 1793
Total pages 9
Place of publication Germany
Publisher Wiley-V C H Verlag
Language eng
Subject 1115 Pharmacology and Pharmaceutical Sciences
Abstract Targeting of vaccines to abundant immune cell populations within our outer thin skin layers using miniaturized devices-much thinner than a needle and syringe, could improve the efficacy of vaccines (and other immunotherapies). To meet this goal, a densely packed dissolving microprojection array (dissolving Nanopatch) is designed, achieving functional miniaturization by 1) formulating small microneedles (two orders of magnitude smaller than a standard needle and syringe) and 2) multiple layering of the payload within microprojections with tight tolerances (of the order of a micrometer). The formulation method is suitable to many vaccines because it is without harsh or complex chemical processes, and it is performed at low temperatures and at a neutral pH. When the formulated dNPs are applied to skin, consistent and robust penetration is achieved, rapidly targeting the skin strata of interest (<5 min; significantly faster than larger dissolving microneedles that have been previously reported). Resultant diffusion is significantly enhanced within the dermis compared with the epidermis. Using two different antigens (ovalbumin and a commercial trivalent influenza vaccine [Fluvax2008]), the administration of these dissolving patches generate robust systemic immune responses in a mouse model. To the authors' knowledge, this is the first report of successful vaccination with any form of dissolving microneedles. The patches made by this method therefore have the potential for pain-free, needle-free, and effective vaccination in humans. © 2010 Wiley-VCH Verlag GmbH and Co. KGaA, Weinheim.
Keyword Drug Delivery
Transdermal Drug-delivery
Polymer Microneedles
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID 569726
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
Australian Institute for Bioengineering and Nanotechnology Publications
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Citation counts: TR Web of Science Citation Count  Cited 67 times in Thomson Reuters Web of Science Article | Citations
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Created: Sun, 19 Sep 2010, 10:01:48 EST