Effects of porcine pre-ovulatory oviductal fluid on boar sperm function

Coy, P., Lloyd, R., Romar, R., Satake, N., Matas, C., Gadea, J. and Holt, W. V. (2010) Effects of porcine pre-ovulatory oviductal fluid on boar sperm function. Theriogenology, 74 4: 632-642. doi:10.1016/j.theriogenology.2010.03.005

Author Coy, P.
Lloyd, R.
Romar, R.
Satake, N.
Matas, C.
Gadea, J.
Holt, W. V.
Title Effects of porcine pre-ovulatory oviductal fluid on boar sperm function
Journal name Theriogenology   Check publisher's open access policy
ISSN 0093-691X
Publication date 2010-09-01
Sub-type Article (original research)
DOI 10.1016/j.theriogenology.2010.03.005
Open Access Status Not Open Access
Volume 74
Issue 4
Start page 632
End page 642
Total pages 11
Place of publication Philadelphia, United States
Publisher Elsevier
Language eng
Formatted abstract
Sperm storage within the oviductal isthmus prior to ovulation typically involves binding to oviductal epithelial cells, which are thought to modulate sperm functions including internal calcium concentration, membrane fluidity, and motility. Around the time of ovulation the spermatozoa are gradually released so that they eventually encounter the oocytes within the oviductal ampulla. Previous studies have shown that the oviductal epithelial cells selectively sequester high quality spermatozoa, but the role of oviductal fluid as a selective modulator of sperm function has been investigated to a lesser extent. Here we address the hypothesis that oviductal fluid is also likely to modulate sperm function. Using samples of porcine oviductal fluid collected in the follicular phase of the estrus cycle, we show that short exposure (20 min to 50 μg/mL of oviductal fluid proteins) to either of two separate proteins fractions (> or < 100 kDa) promotes boar sperm viability and acrosomal integrity, decreases sperm plasma membrane fluidity (measured using merocyanine S540), and increases zona binding and polyspermy during in vitro fertilization. Exposure to the lower molecular fraction significantly inhibited, but did not abolish, the bicarbonate-induced stimulation of motility. The results show that subpopulations of spermatozoa respond differentially to oviductal fluid, and suggest that exposure to oviductal fluid in vivo could exert a further level of functional sperm selection. © 2010 Elsevier Inc.
Keyword Sperm function
Oviductal fluid
Sperm motility
In vitro fertilization
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Agriculture and Food Sciences
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Citation counts: TR Web of Science Citation Count  Cited 22 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 22 times in Scopus Article | Citations
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Created: Sun, 12 Sep 2010, 10:00:44 EST