The natural interleukin-1 receptor antagonist in term and preterm parturition

Romero, R., Sepulveda, W., Mazor, M., Brandt, F., Cotton, D.B., Dinarello, C.A. and Mitchell, M.D. (1992). The natural interleukin-1 receptor antagonist in term and preterm parturition. In: American Journal of Obstetrics and Gynecology:. 12th AnnualL Meeting of The Society of PerinatalL Obstetricians, Orlando , Florida, USA, (863-872). 3-8 February, 1992.

Author Romero, R.
Sepulveda, W.
Mazor, M.
Brandt, F.
Cotton, D.B.
Dinarello, C.A.
Mitchell, M.D.
Title of paper The natural interleukin-1 receptor antagonist in term and preterm parturition
Conference name 12th AnnualL Meeting of The Society of PerinatalL Obstetricians
Conference location Orlando , Florida, USA
Conference dates 3-8 February, 1992
Proceedings title American Journal of Obstetrics and Gynecology:   Check publisher's open access policy
Journal name American Journal of Obstetrics and Gynecology   Check publisher's open access policy
Place of Publication St. Louis, Mo. U.S.A.
Publisher Mosby
Publication Year 1992
Sub-type Fully published paper
ISSN 0002-9378
Volume 167
Issue 4
Start page 863
End page 872
Total pages 9
Language eng
Formatted Abstract/Summary
Objective:
Interleukin-1 has been implicated in the mechanisms responsible for preterm labor in the setting of infection. The interleukin-1 receptor antagonist is a new member of the interleukin-1 gene family that inhibits the biologic effects of interleukin-1 by blocking its receptors. Reduction of interleukin-1-induced prostaglandin production by intrauterine tissues may have potential value in the treatment of preterm labor associated with infection. The purpose of these studies was (1) to determine interleukin-1 receptor antagonist levels in the amniotic fluid of women with term and preterm labor (with and without infection) and (2) to study the effects of interleukin-1 receptor antagonist on interleukin-1-induced prostaglandin biosynthesis by human amnion and chorion.

Study design:
Amniotic fluid was obtained from women in the midtrimester of pregnancy (n = 20), at term pregnancy (with and without labor, n = 69), and in preterm labor (n = 47). Fluid was cultured for aerobic and anaerobic bacteria and Mycoplasmas. Interleukin-1α, interleukin-1β, and interleukin-1 receptor antagonist concentrations were measured by immunoassays previously validated for human amniotic fluid. The effect of interleukin-1 receptor antagonist on interleukin-1-induced prostaglandin production by amnion and chorion was studied with primary cultures. Cells were incubated with interleukin-1 receptor antagonist and interleukin-1α or interleukin-1β for 16 hours. Prostaglandin E2 released into the media was assayed by immunoassay.

Results:
(1) Interleukin-1 receptor antagonist was present in all amniotic fluid samples;
(2) amniotic fluid contains the highest interleukin-1 receptor antagonist concentrations detected in any biologic fluid to date;
(3) amniotic fluid interleukin-1 receptor antagonist concentrations were not increased in women with preterm labor and intraamniotic infection in spite of dramatically elevated concentrations of interleukin-1α and interleukin-1β in the same fluid (median 22 ng/ml and range 0.16 to 70 for preterm labor with negative amniotic fluid culture vs median 30 ng/ml and range 6 to 70 for preterm labor with positive amniotic fluid culture; p > 0.05);
(4) interleukin-1 receptor antagonist reduced interleukin-1β-induced prostaglandin E2 production by amnion and chorion in a dose-dependent manner; (5) interleukin-1 receptor antagonist by itself did not stimulate prostaglandin E2 release by amnion and chorion when used in concentrations ranging from 0.1 to 1000 ng/ml.

Conclusions:
(1) Interleukin-1 receptor antagonist is a physiologic component of amniotic fluid;
(2) the release of interleukin-1α and interleukin-1β into the amniotic fluid in women with preterm labor is not associated with an increase in interleukin-1 receptor antagonist levels in amniotic fluid;
(3) interleukin-1 receptor antagonist reduces interleukin-1-induced prostaglandin production by amnion and chorion;
(4) exogenous anticytokine agents may be of value in the treatment of preterm labor.
Subjects 0604 Genetics
1114 Paediatrics and Reproductive Medicine
Keyword Interleukin-1 Receptor Antagonist
Preterm Labor
Amniotic fluid
Intra-Amniotic Infection
Blood Mononuclear-cells
Prostaglandin Biosynthesis
Inflammatory Mediators
Expression
Invitro
Member
Family
Labor
Q-Index Code E1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Conference Paper
Collection: UQ Centre for Clinical Research Publications
 
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Created: Thu, 26 Aug 2010, 23:12:10 EST