Polyunsaturated fatty acids differentially alter PGF(2 alpha) and PGE(2) release from bovine trophoblast and endometrial tissues during short-term culture

Meiera, S., Ledgard, A.M., Sato, T.A., Peterson, A.J. and Mitchell, M.D. (2009) Polyunsaturated fatty acids differentially alter PGF(2 alpha) and PGE(2) release from bovine trophoblast and endometrial tissues during short-term culture. Animal Reproduction Science, 111 2-4: 353-360. doi:10.1016/j.anireprosci.2008.03.007


Author Meiera, S.
Ledgard, A.M.
Sato, T.A.
Peterson, A.J.
Mitchell, M.D.
Title Polyunsaturated fatty acids differentially alter PGF(2 alpha) and PGE(2) release from bovine trophoblast and endometrial tissues during short-term culture
Journal name Animal Reproduction Science   Check publisher's open access policy
ISSN 0378-4320
1873-2232
Publication date 2009-04-01
Year available 2009
Sub-type Article (original research)
DOI 10.1016/j.anireprosci.2008.03.007
Open Access Status DOI
Volume 111
Issue 2-4
Start page 353
End page 360
Total pages 8
Place of publication Amsterdam, The Netherlands
Publisher Elsevier Science
Language eng
Subject 1103 Clinical Sciences
0606 Physiology
06 Biological Sciences
Abstract Two studies tested the hypothesis that eicosapentaenoic (20:5 omega 3; EPA), docosahexaenoic acids (22:6 omega 3; DHA) or linoleic acid (C18:2 omega 6; LIN) reduced bovine endometrial and trophoblast prostaglandin F-2 alpha. (PGF(2 alpha)) and prostaglandin E-2 (PGE(2)) release during short-term culture. In Study 1, endometrial tissues were collected from non-lactating, non-pregnant cows and endometrial plus trophoblast tissues from pregnant-cows 16 days post-insemination. In Study 2, endometrial and trophoblast tissues were collected on day 17 of pregnancy, from cows synchronised using a double prostaglandin (PG) or Ovagen (TM) synchronisation. Tissues were incubated in medium only (M) or media supplemented with fatty acids: eicosapentaenoic (20:5w3; EPA), docosahexaenoic acids (22:6w3; DHA) or linoleic acid (C18:2 omega 6; LIN). In Study 1, PGE(2) release from 'pregnant' endometria was higher (P=0.094) than from 'non-pregnant' endometria, while PGF(2 alpha) concentrations were similar. Fatty acids treatment had no effect on PGF(2 alpha) or PGE2 release from either pregnant or non-pregnant endometria. Individual fatty acid treatments had no effect on the ratio of PGF(2 alpha) to PGE(2) from trophoblast tissues, but when the data from the 3 fatty acid treatments were combined (EPA, DHA and LIN treatment groups) the ratio of PGF(2 alpha) to PGE(2) was reduced (P = 0.026) when compared to medium only. In Study 2, PGE(2) concentrations were higher (P=0.013) from the trophoblast collected from Ovagen (TM) cows as compared to that of the PG synchrony group. When the data from the 3-omega fatty acids were combined (DHA and EPA treatment groups), the 3-omega treatments decreased (P < 0.05) PGE(2) biosynthesis from both endometrial and trophoblast tissues from animals synchronised following PG synchrony but not Ovagen (TM) synchrony. Short-term culture with low concentrations of 3-omega fatty acids tended to reduce prostaglandin release from trophoblast collected 16 days after insemination, with the type of synchrony modifying PGE(2) production from the trophoblast tissues collected 17 days after insemination. The ability of exogenous fatty acids to modify embryonic prostaglandin release needs to be examined in the context of supplementing dairy cows with different sources of fats. Synchronisation method altered trophoblast PGE(2) release, highlighting the importance of the hormonal environment in modifying embryonic prostaglandin synthesis and release. (c) 2008 Elsevier B.V. All rights reserved.
Formatted abstract
Two studies tested the hypothesis that eicosapentaenoic (20:5ω3; EPA), docosahexaenoic acids (22:6ω3; DHA) or linoleic acid (C18:2ω6; LIN) reduced bovine endometrial and trophoblast prostaglandin F (PGF) and prostaglandin E2 (PGE2) release during short-term culture. In Study 1, endometrial tissues were collected from non-lactating, non-pregnant cows and endometrial plus trophoblast tissues from pregnant cows 16 days post-insemination.In Study 2, endometrial and trophoblast tissues were collected on day 17 of pregnancy, from cows synchronised using a double prostaglandin (PG) or Ovagen™ synchronisation. Tissues were incubated in medium only (M) or media supplemented with fatty acids: eicosapentaenoic (20:5ω3; EPA), docosahexaenoic acids (22:6ω3; DHA) or linoleic acid (C18:2ω6; LIN). In Study 1, PGE2 release from 'pregnant' endometria was higher (P = 0.094) than from 'non-pregnant' endometria, while PGF concentrations were similar. Fatty acids treatment had no effect on PGF or PGE2 release from either pregnant or non-pregnant endometria. Individual fatty acid treatments had no effect on the ratio of PGF to PGE2 from trophoblast tissues, but when the data from the 3 fatty acid treatments were combined (EPA, DHA and LIN treatment groups) the ratio of PGF to PGE2 was reduced (P = 0.026) when compared to medium only.
In Study 2, PGE2 concentrations were higher (P = 0.013) from the trophoblast collected from Ovagen™ cows as compared to that of the PG synchrony group. When the data from the 3-omega fatty acids were combined (DHA and EPA treatment groups), the 3-omega treatments decreased (P < 0.05) PGE2 biosynthesis from both endometrial and trophoblast tissues from animals synchronised following PG synchrony but not Ovagen™ synchrony. Short-term culture with low concentrations of 3-omega fatty acids tended to reduce prostaglandin release from trophoblast collected 16 days after insemination, with the type of synchrony modifying PGE2 production from the trophoblast tissues collected 17 days after insemination. The ability of exogenous fatty acids to modify embryonic prostaglandin release needs to be examined in the context of supplementing dairy cows with different sources of fats. Synchronisation method altered trophoblast PGE2 release, highlighting the importance of the hormonal environment in modifying embryonic prostaglandin synthesis and release. © 2008 Elsevier B.V. All rights reserved.
Keyword Endometrium
Trophoblast
PGF(2 alpha)
PGE(2)
Polyunsaturated fatty acids
Bovine
Prostaglandin Production
Arachidonic-acid
Embryos
Uterine
Expression
Secretion
Ovarian
Cells
Cows
N-3
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID DRCX 0202
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
 
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Created: Thu, 26 Aug 2010, 23:00:41 EST