Use of cDNA arrays to generate differential expression profiles for inflammatory genes in human gestational membranes delivered at term and preterm

Marvin, K.W., Keelan, J.A., Eykholt, R.L., Sato, T.A. and Mitchell, M.D. (2002) Use of cDNA arrays to generate differential expression profiles for inflammatory genes in human gestational membranes delivered at term and preterm. Molecular Human Reproduction, 8 4: 399-408. doi:10.1093/molehr/8.4.399


Author Marvin, K.W.
Keelan, J.A.
Eykholt, R.L.
Sato, T.A.
Mitchell, M.D.
Title Use of cDNA arrays to generate differential expression profiles for inflammatory genes in human gestational membranes delivered at term and preterm
Journal name Molecular Human Reproduction   Check publisher's open access policy
ISSN 1360-9947
1360-9947
Publication date 2002-04-01
Sub-type Article (original research)
DOI 10.1093/molehr/8.4.399
Volume 8
Issue 4
Start page 399
End page 408
Total pages 10
Place of publication Oxford, England
Publisher Published for the European Society for Human Reproduction and Embryology by Oxford University Press
Language eng
Subject 1103 Clinical Sciences
1114 Paediatrics and Reproductive Medicine
1115 Pharmacology and Pharmaceutical Sciences
Formatted abstract
Inflammatory processes are implicated in preterm labour (PTL). To identify potential novel markers for PTL, we have used commercial cDNA arrays to generate profiles of differential expression of inflammation-associated genes in gestational membranes with term and PTL. RNA for cDNA probe synthesis was isolated from reflected human amnion and choriodecidua membranes delivered following Caesarean section at term before the onset of labour (TNL, n = 4), spontaneous labour at term (TSL, n = 4), and PTL with and without chorioamnionitis (PTL+INF and PTL-INF respectively, n = 4 each). Profiles were displayed relative to TNL and statistical comparisons of TSL versus TNL and PTL+INF versus PTL-INF were performed. Elevated expression of chemokines macrophage inflammatory protein 1β(MIP-1β) and pulmonary and activation-regulated chemokine (PARC) was observed in PTL+INF compared to PTL-INF amnion and choriodecidua respectively (P = 0.03). Likewise, the cytokines oncostatin-M and pre-B cell enhancing factor (PBEF) were more highly expressed in PTL+INF compared with PTL-INF and in TSL compared with TNL respectively (P = 0.03). Conversely, inhibin A, tissue inhibitors of matrix metalloproteinase (TIMP)-3 and TIMP-4 were all significantly elevated in PTL-INF compared with PTL+INF (P = 0.03). Furthermore, differential expression patterns of classes of genes, grouped according to function (e.g. chemokines), were noted. The cDNA array approach holds promise for identification of new candidate markers or combinations thereof for prediction or diagnosis of PTL, as well as for increasing our understanding of the particular aetiologies involved.
Keyword Amnion
Chorioamnionitis
Cytokines
Labour
Preterm labour
Aminiotic-Fluid Interleukin-6
Adhesion Molecule-1 Icam-1
Pro-Matrix Metalloproteinase-9
Prostaglandin E(2) Production
Uterine Cervical Fibroblasts
Stimulating Factor Levels
Necrosis-factor-alpha
Microbial Invasion
Intrauterine Infection
Intact Membranes
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
 
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Created: Thu, 26 Aug 2010, 22:59:50 EST