Characterization of the endocannabinoid system in early human pregnancy

Helliwell, Rachel J. A., Chamley, Lawrence W., Blake-Palmer, Katherine, Mitchell, Murray D., Wu, Janice, Kearn, Christopher S. and Glass, Michelle (2004) Characterization of the endocannabinoid system in early human pregnancy. Journal of Clinical Endocrinology & Metabolism, 89 10: 5168-5174. doi:10.1210/jc.2004-0388

Author Helliwell, Rachel J. A.
Chamley, Lawrence W.
Blake-Palmer, Katherine
Mitchell, Murray D.
Wu, Janice
Kearn, Christopher S.
Glass, Michelle
Title Characterization of the endocannabinoid system in early human pregnancy
Journal name Journal of Clinical Endocrinology & Metabolism   Check publisher's open access policy
ISSN 0021-972X
Publication date 2004-10-01
Sub-type Article (original research)
DOI 10.1210/jc.2004-0388
Volume 89
Issue 10
Start page 5168
End page 5174
Total pages 7
Place of publication Bethesda, MD U.S.A.
Publisher Endocrine Society
Language eng
Subject 0604 Genetics
1103 Clinical Sciences
Abstract In recent years, it has been demonstrated that high circulating levels of the endogenous cannabinoid anandamide, resulting from low expression of its metabolizing enzyme fatty acid amide hydrolase (FAAH), may contribute to spontaneous miscarriage and poor outcome in women undergoing in vitro fertilization. The site of action of this compound, however, has not been determined. In this study, we examined the distribution of the cannabinoid receptors, CB1 and CB2, and the endocannabinoid-metabolizing enzyme FAAH in first trimester human placenta. Here, we show that FAAH is expressed throughout the human first trimester placenta, in extravillous trophoblast columns, villous cytotrophoblasts, syncytiotrophoblasts, and macrophages. Furthermore, FAAH mRNA levels appear to be regulated during gestation, with levels peaking at 11 wk before declining again. The immune system-associated cannabinoid CB2 receptors were localized only to placental macrophages. Interestingly, the cannabinoid receptor CB1 was not identified in first trimester placenta despite having previously been shown to be present in placental tissues at term. These findings suggest that the placenta may form a barrier preventing maternal-fetal transfer of anandamide and/or modulate local levels of anandamide by regulation of FAAH expression with gestation.
Keyword Acid Amide Hydrolase
Cannabinoid Receptors
Anandamide Synthesis
Embryo Implantation
Uterine Receptivity
Mouse Uterus
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
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Created: Thu, 26 Aug 2010, 22:54:51 EST