Transcriptional regulation of prostaglandin-H-synthase-1 in the amnion-derived AV3 cell line

Eykholt, R.L., Potter, S., Marvin, K.W. and Mitchell, M.D. (2001) Transcriptional regulation of prostaglandin-H-synthase-1 in the amnion-derived AV3 cell line. Prostaglandins and Other Lipid Mediators, 63 3: 123-131. doi:10.1016/S0090-6980(00)00102-7

Author Eykholt, R.L.
Potter, S.
Marvin, K.W.
Mitchell, M.D.
Title Transcriptional regulation of prostaglandin-H-synthase-1 in the amnion-derived AV3 cell line
Journal name Prostaglandins and Other Lipid Mediators   Check publisher's open access policy
ISSN 1098-8823
Publication date 2001-01-01
Sub-type Article (original research)
DOI 10.1016/S0090-6980(00)00102-7
Volume 63
Issue 3
Start page 123
End page 131
Total pages 9
Place of publication New York, N.Y. U.S.A.
Publisher Elsevier Science
Language eng
Subject 1103 Clinical Sciences
1114 Paediatrics and Reproductive Medicine
1115 Pharmacology and Pharmaceutical Sciences
Formatted abstract
Prostaglandin-H-synthase-1 (PGHS-1), while constitutively expressed in most tissues, increases in abundance in human gestational membranes at term. This suggests that PGHS-1 may be up-regulated in preparation for labor, and thus might be a key determinant in timing labor onset. We conducted transient transfection experiments in amnion-derived AV3 cells utilizing pPGHS1CAT to identify substances that might regulate PGHS-1 expression in amnion. Transforming growth factor-β (1 ng/ml) and 15-deoxy-Δ12,14 prostaglandin J2 (1μM) significantly (P < 0.05) (33% and 44% respectively) increased PGHS-1 promoter activity. The activity decreased significantly (P < 0.05) in response to interleukin-1 (IL-1)β (1 ng/ml) (45%), tumor necrosis factor (TNF)-α (50 ng/ml) (34%), epidermal growth factor (10 ng/ml) (54%), phorbol myristate acetate (10 nM) (70%), IL-4 (10 ng/ml) (50%), IL-8 (100 ng/ml) (72%) and Activin A (25 ng/ml) (32%). Whether this degree of change in promoter activity leads to physiologically relevant alterations in the amounts of PGHS-1 present in cells remains to be determined. © 2001 Elsevier Science Inc.
Keyword Tumor-necrosis-factor
Osteoblastic Mc3t3-e1 Cells
Pro-Inflammatory Cytokines
Human Fetal Membranes
Endoperoxide Synthase
Human Monocytes
Preterm Labor
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
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Created: Thu, 26 Aug 2010, 22:52:35 EST