5-Hydroxyeicosatetraenoic acid and human parturition

Edwin, S. S., Romero, R. J., Munoz, H., Branch, D. W. and Mitchell, M. D. (1996) 5-Hydroxyeicosatetraenoic acid and human parturition. Prostaglandins, 51 6: 403-412. doi:10.1016/0090-6980(96)00046-9


Author Edwin, S. S.
Romero, R. J.
Munoz, H.
Branch, D. W.
Mitchell, M. D.
Title 5-Hydroxyeicosatetraenoic acid and human parturition
Journal name Prostaglandins   Check publisher's open access policy
ISSN 0090-6980
Publication date 1996-06-01
Year available 1996
Sub-type Article (original research)
DOI 10.1016/0090-6980(96)00046-9
Open Access Status Not yet assessed
Volume 51
Issue 6
Start page 403
End page 412
Total pages 10
Place of publication Stoneham, Ma., U.S.A.
Publisher Butterworths
Language eng
Subject 0604 Genetics
1114 Paediatrics and Reproductive Medicine
Abstract 5-Hydroxyeicosatetraenoic acid (5-HETE) is an arachidonic acid (AA) metabolite derived from the lipoxygenase pathway which is capable of inducing uterine contractions. The purpose of this study was to determine a). whether 5-HETE concentrations in amniotic fluid increase before or after the onset of labor and b). whether acetylsalicylic acid (ASA) could modulate the production of 5-HETE by human amnion cells. 5-HETE concentrations are increased in amniotic fluid before the onset of labor. Furthermore, ASA treatment as expected inhibited PGE(2), but also significantly increased 5-HETE production by amnion cells. 5-HETE concentrations on average increased by greater than 2.5 fold (p < 0.001) in amniotic fluid prior to spontaneous labor when compared with samples obtained from the same patients earlier in gestation and therefore may be important in mechanisms regulating the onset of labor. ASA provokes an increase in 5-HETE biosynthesis by amnion cells: control media 2.60 +/- 1.5, ASA treatment alone 5.17 +/- 0.20, IL-1 beta alone 6.39 +/- 2.1, and ASA + IL-1 beta 8.95 +/- 1.2 (mean +/- SEM) picograms per microgram protein per 16 hours. These findings may explain in part why cyclooxygenase inhibitors are not always successful in treating women with preterm labor.
Formatted abstract
5-Hydroxyeicosatetraenoic acid (5-HETE) is an arachidonic acid (AA) metabolite derived from the lipoxygenase pathway which is capable of inducing uterine contractions. The purpose of this study was to determine a), whether 5-HETE concentrations in amniotic fluid increase before or after the onset of labor and b), whether acetylsalicylic acid (ASA) could modulate the production of 5-HETE by human amnion cells. 5-HETE concentrations are increased in amniotic fluid before the onset of labor. Furthermore, ASA treatment as expected inhibited PGE2, but also significantly increased 5- HETE production by amnion cells. 5-HETE concentrations on average increased by greater than 2.5 fold (p < 0.001) in amniotic fluid prior to spontaneous labor when compared with samples obtained from the same patients earlier in gestation and therefore may be important in mechanisms regulating the onset of labor. ASA provokes an increase in 5-HETE biosynthesis by amnion cells: control media 2.60 ± 1.5, ASA treatment alone 5.17 ± 0.20, IL-1β alone 6.39 ± 2.1, and ASA + IL-1β 8.95 ± 1.2 (mean ± SEM) picograms per microgram protein per 16 hours. These findings may explain in part why cyclooxygenase inhibitors are not always successful in treating women with preterm labor.
Keyword 5-Hydroxyeicosatetraenoic Acid
Interleukin-1 beta
Amniotic fluid
Acetylsalicylic acid
Arachidonate Lipoxygenase Metabolites
Amniotic-fluid
Preterm Labor
Fetal Membranes
Cells
Infection
Biosynthesis
Tissues
Calcium
Onset
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID HD20747
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
 
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Created: Thu, 26 Aug 2010, 22:51:59 EST