Actions of immunoregulatory agents on 5-HETE production by cultured human amnion cells

Edwin, S.S., Mitchell, M.D. and Dudley, D.J. (1997) Actions of immunoregulatory agents on 5-HETE production by cultured human amnion cells. Journal of Reproductive Immunology, 36 1-2: 111-121. doi:10.1016/S0165-0378(97)00064-8

Author Edwin, S.S.
Mitchell, M.D.
Dudley, D.J.
Title Actions of immunoregulatory agents on 5-HETE production by cultured human amnion cells
Journal name Journal of Reproductive Immunology   Check publisher's open access policy
ISSN 0165-0378
Publication date 1997-11-01
Sub-type Article (original research)
DOI 10.1016/S0165-0378(97)00064-8
Volume 36
Issue 1-2
Start page 111
End page 121
Total pages 11
Place of publication Amsterdam, The Netherlands
Publisher Elsevier Science
Language eng
Subject 1103 Clinical Sciences
1114 Paediatrics and Reproductive Medicine
1115 Pharmacology and Pharmaceutical Sciences
Formatted abstract
Labor in women is associated with increased amniotic fluid prostaglandin (PG) concentrations. AF of women with term and preterm labor also contains elevated concentrations of arachidonic acid metabolites from the lipoxygenase pathway including 5-hydroxyeicosatetranoic acid (5-HETE). We and others have shown that 5-HETE production within the uterus is increased by inflammatory cytokines. The purpose of this study was to determine whether 5-HETE production is regulated by immunotherapeutic agents that inhibit prostaglandin E2 (PGE2) production, such as interleukin-10 (IL-10) and cyclosporin A (CsA). Cultured confluent amnion cells were incubated for 16 h with IL-10 (1-100 ng/ml), CsA (1-1000 ng/ml), or controls. Additional incubations of IL-10, and CsA (both at 10 and 100 ng/ml) were conducted on amnion cells in the presence and absence of interleukin-1β at 1 ng/ml. 5-HETE was measured by radioimmunoassay and cellular protein determined. IL-1β stimulated amnion cell 5-HETE production as expected. However, 5-HETE production by amnion cells was significantly inhibited by incubation with IL-10 and CsA, and both significantly attenuated 5-HETE production in response to IL-1β. We speculate that the inhibitory effect of IL-10 and CsA on amnion cell 5-HETE production is at the level of substrate release or inhibition of 5-lipoxygenase and that the use of these agents during pregnancy may not adversely affect pregnancy outcomes.
Keyword Amnion cells
Cyclosporin A
Interleukin-1 beta
Arachidonate Lipoxygenase Metabolites
Inhibits Cytokine Production
Renal-Transplant Recipient
Preterm Labor
5-Hydroxyeicosatetraenoic Acid
Successful Pregnancy
Human Parturition
IL-10 Production
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
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Created: Thu, 26 Aug 2010, 22:51:53 EST