The osteoblast-like differentiated phenotype of a variant of MG-63 osteosarcoma cell line correlated with altered adhesive properties

Dedhar, Shoukat, Mitchell, Murray D. and Pierschbacher, Michael D. (1989) The osteoblast-like differentiated phenotype of a variant of MG-63 osteosarcoma cell line correlated with altered adhesive properties. Connective Tissue Research, 20 1-4: 49-61. doi:10.3109/03008208909023874


Author Dedhar, Shoukat
Mitchell, Murray D.
Pierschbacher, Michael D.
Title The osteoblast-like differentiated phenotype of a variant of MG-63 osteosarcoma cell line correlated with altered adhesive properties
Journal name Connective Tissue Research   Check publisher's open access policy
ISSN 0300-8207
1521-0456
Publication date 1989-01-01
Year available 1989
Sub-type Article (original research)
DOI 10.3109/03008208909023874
Open Access Status Not yet assessed
Volume 20
Issue 1-4
Start page 49
End page 61
Total pages 13
Place of publication Philadelphia, PA., U.S.A.
Publisher Taylor and Francis
Language eng
Subject 1103 Clinical Sciences
1112 Oncology and Carcinogenesis
Abstract A cell line, called MG-63.3A, was selected for its resistance to detachment from cell culture by a synthetic peptide containing the fibronectin cell-attachment sequence, Arg-Gly-Asp-Ser. The mechanism of this resistance is probably the 6-fold overproduction of the cell surface fibronectin receptor in MG-63.3A cells (Dedhar et al, J. Cell. Biol. 105, 1175-1182, (1987]. Compared to the parental, tumorigenic MG-63 cells, the non-tumorigenic MG-63.3A cells display strikingly different properties. These include an altered morphology, a slower proliferation rate, ability to form a calcified matrix in vitro, increased synthesis of type I collagen and expression of bone type alkaline phosphatase activity. Studies with purified growth factors indicate that the MG-63 and MG-63.3A cell lines respond to differentially to growth factors; the growth of MG-63 cells if stimulated by PDGF and GM-CSF and inhibited IL-1 beta, whereas the growth of MG-63.3A is unaffected by GM-CSF and IL-1 beta but is stimulated by PDGF and estradiol. We conclude from these data that the MG-63.3A cells may represent a more differentiated cell type with osteoblast-like properties. Studies are currently underway to further characterize, by electron microscopy, the calcified matrix formation by MG-63.3A cells.
Keyword Osteosarcoma
Osteoblast-like Cells
Integrins
Cytokines
Prostaglandins
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID CA 28896
HD 20779
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
 
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Created: Thu, 26 Aug 2010, 22:50:27 EST