Conformationally altered beta(2)-glycoprotein I is the antigen for anti-cardiolipin autoantibodies

Chamley, L.W., Duncalf, A.M., Konarkowska, B., Mitchell, M.D. and Johnson, P.M. (1999) Conformationally altered beta(2)-glycoprotein I is the antigen for anti-cardiolipin autoantibodies. Clinical And Experimental Immunology, 115 3: 571-576. doi:10.1046/j.1365-2249.1999.00810.x

Author Chamley, L.W.
Duncalf, A.M.
Konarkowska, B.
Mitchell, M.D.
Johnson, P.M.
Title Conformationally altered beta(2)-glycoprotein I is the antigen for anti-cardiolipin autoantibodies
Journal name Clinical And Experimental Immunology   Check publisher's open access policy
ISSN 0009-9104
Publication date 1999-03-01
Sub-type Article (original research)
DOI 10.1046/j.1365-2249.1999.00810.x
Volume 115
Issue 3
Start page 571
End page 576
Total pages 6
Place of publication Oxford, England
Publisher Blackwell Scientific Publications
Language eng
Subject 1103 Clinical Sciences
1115 Pharmacology and Pharmaceutical Sciences
Formatted abstract
Anti-cardiolipin autoantibodies (aCL) induce thrombosis and recurrent fetal death. These antibodies require a 'cofactor', identified as β2- glycoprotein I (β2-GPI), to bind phospholipids. We show here that aCL can bind β2-GPI in the absence of phospholipid. Binding of aCL to β2-GPI is dependent upon the β2-GPI being immobilized on an appropriate surface including cardiolipin, irradiated polystyrene and nitrocellulose membrane. This effect cannot be explained by increased antigen density of β2-GPI immobilized on these surfaces. Rather, conformational changes that occur following the interaction of β2-GPI with phospholipid render this protein antigenic to aCL. Liquid-phase β2-GPI was not antigenic for aCL. Thus, aCL cannot bind circulating β2-GPI. These findings may explain why patients with aCL can remain healthy for many years but then undergo episodes of thrombosis or fetal loss without changes in their circulating aCL profile, as the triggering event for these pathologies can be predicted to be one that renders β2-GPI antigenic for aCL.
Keyword Beta(2)-glycoprotein I
Antigen binding autoimmunity
Systemic Lupus-erythematosus
Beta-2-Glycoprotein-I Apolipoprotein-H
Antiphospholipid Antibodies
Anticardolipin Antibodies
Recognize Beta(2)-Glycoprotein-I
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
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Created: Thu, 26 Aug 2010, 22:49:18 EST