Immunoglobulin G fractions from patients with antiphospholipid antibodies cause fetal death in BALB/c mice: A model for autoimmune fetal loss

Branch, D. W., Dudley, D. J., Mitchell, M. D., Creighton, K. A., Abbott, T. M., Hammond, E. H. and Daynes, R. A. (1990) Immunoglobulin G fractions from patients with antiphospholipid antibodies cause fetal death in BALB/c mice: A model for autoimmune fetal loss. American Journal of Obstetrics and Gynecology, 163 1: 210-216.

Author Branch, D. W.
Dudley, D. J.
Mitchell, M. D.
Creighton, K. A.
Abbott, T. M.
Hammond, E. H.
Daynes, R. A.
Title Immunoglobulin G fractions from patients with antiphospholipid antibodies cause fetal death in BALB/c mice: A model for autoimmune fetal loss
Journal name American Journal of Obstetrics and Gynecology   Check publisher's open access policy
ISSN 0002-9378
Publication date 1990-07-01
Sub-type Article (original research)
Volume 163
Issue 1
Start page 210
End page 216
Total pages 7
Place of publication St. Louis, Mo., U. S. A.
Publisher Mosby
Language eng
Subject 1114 Paediatrics and Reproductive Medicine
Abstract We determined whether purified immunoglobulin G from patients with antiphospholipid antibodies causes fetal loss in pregnant mice. Sera were obtained from nonpregnant parous women (group 1) and nonpregnant women with antiphospholipid antibodies and a history of fetal loss (group 2). Pregnant BALB/c mice were given an intraperitoneal injection of 15 mg of IgG on day 8 of pregnancy. Typically, mice treated with IgG from antiphospholipid antibodies aborted within 48 hours. When animals were sacrificed on days 9 to 15, the uterus of each animal was inspected for the presence of live, dead, or resorbing fetuses. In contrast to mice injected with control IgG or saline solution, each mouse injected with IgG from antiphospholipid antibodies aborted and no live fetuses were found (p < 0.05). Histologic examination of the uteroplacental interface showed decidual necrosis in the mice treated with IgG containing antiphospholipid antibodies, and immunofluorescent studies also showed prominent intravascular decidual IgG and fibrin deposition. We conclude that IgG from antiphospholipid antibodies of women with fetal loss causes fetal loss in BALB/c mice. It appears that the fetal loss is mediated by IgG binding in the maternal decidual vasculature.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 253 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 263 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Thu, 26 Aug 2010, 22:48:14 EST