Hypoxia inhibits activin A production by term villous trophoblast in vitro

Blumenstein, M., Mitchell, M. D., Groome, N. P. and Keelan, J. A. (2002) Hypoxia inhibits activin A production by term villous trophoblast in vitro. Placenta, 23 10: 735-741. doi:10.1053/plac.2002.0868

Author Blumenstein, M.
Mitchell, M. D.
Groome, N. P.
Keelan, J. A.
Title Hypoxia inhibits activin A production by term villous trophoblast in vitro
Journal name Placenta   Check publisher's open access policy
ISSN 0143-4004
Publication date 2002-11-01
Sub-type Article (original research)
DOI 10.1053/plac.2002.0868
Volume 23
Issue 10
Start page 735
End page 741
Total pages 7
Place of publication London, England
Publisher W.B. Saunders
Language eng
Subject 1103 Clinical Sciences
1114 Paediatrics and Reproductive Medicine
Abstract Elevated activin A and inhibin A levels have been associated with pre-eclampsia, a pregnancy-related disorder associated with placental hypoxaemia. We investigated the effect of in vitro hypoxia on the production of inhibin A, activin A and its binding protein follistatin in term villous placental explants (n = 4-7) and trophoblast monolayer cultures (n = 4). Explants and trophoblasts were incubated for 24-72 h under either normoxic (21 per cent O2) or hypoxic (2 per cent O2) conditions. Production of activin A, inhibin A, and follistatin was determined by specific ELISA. After 48 h of hypoxia, villous explants exhibited a significant reduction in activin A production rates to 53.2 ± 8.9 per cent (mean ± SEM, P<0.05) of normoxic controls which was sustained after 72 h in culture (46.8 ± 5.9 per cent), whereas production by trophoblast monolayers was not affected by hypoxia. Follistatin production was decreased to 53.7 ± 9.2 per cent of control (P<0.05) after 48 h of hypoxia. Inhibin A production remained unaltered in both culture systems. Our data demonstrate for the first time that hypoxia lowers term placental activin A and follistatin production in vitro. These findings do not support the notion that elevated circulating activin A levels in pre-eclampsia originate from the placenta as a result of placental hypoxia. Other as yet unknown maternal/placental factors may contribute to elevated activin A production in women with severe pre-eclampsia. © 2002 Elsevier Science Ltd. All rights reserved.
Keyword 2-site Enzyme-Immunoassay
Human Placenta
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
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Citation counts: TR Web of Science Citation Count  Cited 19 times in Thomson Reuters Web of Science Article | Citations
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Created: Thu, 26 Aug 2010, 22:47:02 EST