Tomato yellow leaf curl virus in Australia: distribution, detection and discovery of naturally occurring defective DNA molecules

Van Brunschot, S. L., Persley, D. M., Geering, A. D. W., Campbell, P. R. and Thomas, J. E. (2010) Tomato yellow leaf curl virus in Australia: distribution, detection and discovery of naturally occurring defective DNA molecules. Australasian Plant Pathology, 39 5: 412-423. doi:10.1071/AP10083


Author Van Brunschot, S. L.
Persley, D. M.
Geering, A. D. W.
Campbell, P. R.
Thomas, J. E.
Title Tomato yellow leaf curl virus in Australia: distribution, detection and discovery of naturally occurring defective DNA molecules
Formatted title
Tomato yellow leaf curl virus in Australia: distribution, detection and discovery of naturally occurring defective DNA molecules
Journal name Australasian Plant Pathology   Check publisher's open access policy
ISSN 0815-3191
1448-6032
Publication date 2010-08-01
Sub-type Article (original research)
DOI 10.1071/AP10083
Open Access Status Not Open Access
Volume 39
Issue 5
Start page 412
End page 423
Total pages 12
Place of publication Collingwood, Vic., Australia
Publisher CSIRO
Language eng
Subject C1
820215 Vegetables
060704 Plant Pathology
Abstract Tomato yellow leaf curl virus (TYLCV) seriously impacts tomato production throughout tropical and sub-tropical regions of the world. It has a broad geographical distribution and continues to spread to new regions in the Indian and Pacific Oceans including Australia, New Caledonia and Mauritius. We undertook a temporally-scaled, phylogeographic analysis of all publicly available, full genome sequences of TYLCV, together with 70 new genome sequences from Australia, Iran and Mauritius. This revealed that whereas epidemics in Australia and China likely originated through multiple independent viral introductions from the East-Asian region around Japan and Korea, the New Caledonian epidemic was seeded by a variant from the Western Mediterranean region and the Mauritian epidemic by a variant from the neighbouring island of Reunion. Finally, we show that inter-continental scale movements of TYLCV to East Asia have, at least temporarily, ceased, whereas long-distance movements to the Americas and Australia are probably still ongoing.
Formatted abstract
Tomato yellow leaf curl virus (TYLCV) was detected for the first time in Australia in March 2006 in field-grown tomatoes in Brisbane, Queensland. Surveys showed that the virus was confined to south-east Queensland. Virus transmission studies carried out using Bemisia tabaci (B biotype) verified that resistant tomato lines containing the Ty-1 or Ty-5 genes displayed tolerance to infection by TYLCV isolates from Australia. A PCR assay specific for TYLCV was designed and optimised to confirm the presence of the virus in samples that tested positive in begomovirus-specific double-antibody sandwich enzyme-linked immunosorbent assay. Eight isolates of TYLCV from various sites were cloned and sequenced, and were shown to have near-identical sequences and a high nucleotide sequence similarity (≥98%) to the monopartite Tomato yellow leaf curl virus-Israel (TYLCV-IL). No DNA-B, DNA-1 nor DNA-β satellite molecules were detected using degenerate PCR assays. Phylogenetic analysis revealed that Australian isolates of TYLCV separated into two sequence groups, TYLCV-IL[Au:Bri:06] and TYLCV-IL[Au:Bun:06], that showed a defined geographic segregation. Naturally occurring defective DNA molecules containing partial, rearranged segments of the native DNA-A, were present in one isolate. To our knowledge, this is the first report of an incursion of a begomovirus into Australia, and the first report of the characterisation of naturally occurring defective DNAs of TYLCV.
© 2010 Australasian Plant Pathology Society.

Keyword Diagnostics
Geminiviridae
Rolling-circle amplification
Virus host range
Whitefly transmitted geminiviruses
Pcr mediated amplification
Universal primers
Interfering DNAs
Resistance level
Beta complexes
Plant viruses
Begomovirus
Satellite
Disease
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
Queensland Alliance for Agriculture and Food Innovation
School of Biological Sciences Publications
 
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Created: Sun, 15 Aug 2010, 10:07:14 EST