Transcriptional repression by COUP-TF II is dependent on the C-terminal domain and involves the N-CoR variant, RIP13delta1

Bailey, Peter J., Dowhan, Dennis H., Franke, Katrina, Burke, Les J., Downes, Michael and Muscat, George E. O. (1997) Transcriptional repression by COUP-TF II is dependent on the C-terminal domain and involves the N-CoR variant, RIP13delta1. The Journal of Steroid Biochemistry and Molecular Biology, 63 4-6: 165-174. doi:10.1016/S0960-0760(97)00079-4


Author Bailey, Peter J.
Dowhan, Dennis H.
Franke, Katrina
Burke, Les J.
Downes, Michael
Muscat, George E. O.
Title Transcriptional repression by COUP-TF II is dependent on the C-terminal domain and involves the N-CoR variant, RIP13delta1
Formatted title
Transcriptional repression by COUP-TF II is dependent on the C-terminal domain and involves the N-CoR variant, RIP13δ1
Journal name The Journal of Steroid Biochemistry and Molecular Biology   Check publisher's open access policy
ISSN 0960-0760
Publication date 1997-01-01
Sub-type Article (original research)
DOI 10.1016/S0960-0760(97)00079-4
Open Access Status Not Open Access
Volume 63
Issue 4-6
Start page 165
End page 174
Total pages 10
Place of publication Oxford, UK
Publisher Elsevier Science
Language eng
Subject 060199 Biochemistry and Cell Biology not elsewhere classified
Formatted abstract
COUP-TF II/ARP-1 is an ‘orphan’ steroid receptor that inhibits basal transcription, and represses trans-activation by the vitamin D, thyroid hormone and retinoid receptors. The molecular basis of repression by COUP-TF II remains obscure. In this study we utilized the GAL4 hybrid system to demonstrate that COUP-TF II contains sequences within the C-terminal region that encode a dominant transcriptional repressor that inhibits the ability of the potent chimeric transactivator GAL4VP16 to induce transcription. Mammalian two hybrid analysis demonstrated that COUP-TF II did not efficiently interact with either interaction domains I or II from N-CoR and RIP13. However, COUP-TF II efficiently interacts with a region comprised of interaction domains I + II from the corepressor, RIP13δ1. Efficient interaction of the orphan receptor with the corepressor was critically dependent on a large region comprised of the C, D and E domains of COUP-TF II, which correlated with the domain that maximally represses transcription. This investigation suggested that the N-CoR variant, RIP13δ1 interacts with a region of COUP-TF II that functions as a dominant transcriptional repressor.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Diamantina Institute Publications
 
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Created: Mon, 19 Jul 2010, 22:28:39 EST by Laura McTaggart on behalf of UQ Diamantina Institute