Ectopic expression of an endoparasitic wasp venom protein in Drosophila melanogaster affects immune function, larval development and oviposition

Thomas, P., Yamada, R., Johnson, K. N. and Asgari, S. (2010) Ectopic expression of an endoparasitic wasp venom protein in Drosophila melanogaster affects immune function, larval development and oviposition. Insect Molecular Biology, 19 4: 473-480. doi:10.1111/j.1365-2583.2010.01004.x


Author Thomas, P.
Yamada, R.
Johnson, K. N.
Asgari, S.
Title Ectopic expression of an endoparasitic wasp venom protein in Drosophila melanogaster affects immune function, larval development and oviposition
Formatted title
Ectopic expression of an endoparasitic wasp venom protein in Drosophila melanogaster affects immune function, larval development and oviposition
Journal name Insect Molecular Biology   Check publisher's open access policy
ISSN 0307-6978
0962-1075
1365-2583
Publication date 2010-08-01
Sub-type Article (original research)
DOI 10.1111/j.1365-2583.2010.01004.x
Open Access Status Not Open Access
Volume 19
Issue 4
Start page 473
End page 480
Total pages 8
Editor Linda M. Field
David A. O'Brochta
Place of publication Oxford, U.K.
Publisher Blackwell Scientific for the Royal Entomological Society
Language eng
Subject C1
970106 Expanding Knowledge in the Biological Sciences
060808 Invertebrate Biology
Formatted abstract
Endoparasitic hymenoptera inject maternal factors into the host, along with their eggs, to subvert the host immune system. The venom protein, Vn50, previously characterized from the wasp Cotesia rubecula inhibits prophenoloxidase activation in its host Pieris rapae and in another lepidopteran, Manduca sexta. We generated a stable line in the model insect, Drosophila melanogaster, which ectopically expresses Vn50. Results indicated that Vn50 expression accelerates larval development, increases oviposition and reduces melanization in the haemolymph of the transgenic flies. Since melanization is known to be an important facet of the insect immune response, we examined the impact of Vn50 expression on susceptibility to pathogens. Transgenic Vn50 flies challenged with the fungus Beauveria bassiana had increased mortality compared with control flies, but there was no significant change in survival in flies challenged with the pathogenic bacteria, Serratia marcescens. Interestingly, mortality induced by the natural pathogen Drosophila C virus was significantly delayed in Vn50 expressing flies. This indicates a wider range of potential hosts that may be affected by Vn50 and its potential for manipulation of immune system in insects.
© 2010 The Royal Entomological Society.

Keyword Drosophila melanogaster
Vn50
Prophenoloxidase
Melanization
Immunity
Serine protease homologue
Dopa decarboxylase
Innate immunity
Ssystem
Apterous(56F)
Protection
Activation
Mechanism
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Biological Sciences Publications
 
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Created: Sun, 18 Jul 2010, 10:06:05 EST