Escherichia coli isolates causing asymptomatic bacteriuria in catheterized and noncatheterized individuals possess similar virulence properties

Watts, Rebecca E., Hancock, Viktoria, Ong, Cheryl-Lynn Y., Vejborg, Rebecca Munk, Mabbett, Amanda N., Totsika, Makrina, Looke, David F., Nimmo, Graeme R., Klemm, Per and Schembri, Mark A. (2010) Escherichia coli isolates causing asymptomatic bacteriuria in catheterized and noncatheterized individuals possess similar virulence properties. Journal of Clinical Microbiology, 48 7: 2449-2458. doi:10.1128/JCM.01611-09

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Author Watts, Rebecca E.
Hancock, Viktoria
Ong, Cheryl-Lynn Y.
Vejborg, Rebecca Munk
Mabbett, Amanda N.
Totsika, Makrina
Looke, David F.
Nimmo, Graeme R.
Klemm, Per
Schembri, Mark A.
Title Escherichia coli isolates causing asymptomatic bacteriuria in catheterized and noncatheterized individuals possess similar virulence properties
Formatted title
Escherichia coli isolates causing asymptomatic bacteriuria in catheterized and noncatheterized individuals possess similar virulence properties
Journal name Journal of Clinical Microbiology   Check publisher's open access policy
ISSN 0095-1137
1098-660X
1070-633X
Publication date 2010-07-01
Sub-type Article (original research)
DOI 10.1128/JCM.01611-09
Open Access Status File (Publisher version)
Volume 48
Issue 7
Start page 2449
End page 2458
Total pages 10
Editor Gary V. Doern
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Formatted abstract
Urinary tract infections (UTIs) are among the most common infectious diseases of humans, with Escherichia coli being responsible for >80% of all cases. Asymptomatic bacteriuria (ABU) occurs when bacteria colonize the urinary tract without causing clinical symptoms and can affect both catheterized patients (catheter-associated ABU [CA-ABU]) and noncatheterized patients. Here, we compared the virulence properties of a collection of ABU and CA-ABU nosocomial E. coli isolates in terms of antibiotic resistance, phylogenetic grouping, specific UTI-associated virulence genes, hemagglutination characteristics, and biofilm formation. CA-ABU isolates were similar to ABU isolates with regard to the majority of these characteristics; exceptions were that CA-ABU isolates had a higher prevalence of the polysaccharide capsule marker genes kpsMT II and kpsMT K1, while more ABU strains were capable of mannose-resistant hemagglutination. To examine biofilm growth in detail, we performed a global gene expression analysis with two CA-ABU strains that formed a strong biofilm and that possessed a limited adhesin repertoire. The gene expression profile of the CA-ABU strains during biofilm growth showed considerable overlap with that previously described for the prototype ABU E. coli strain, 83972. This is the first global gene expression analysis of E. coli CA-ABU strains. Overall, our data suggest that nosocomial ABU and CA-ABU E. coli isolates possess similar virulence profiles.
Copyright © 2010, American Society for Microbiology. All Rights Reserved.
Keyword Urinary-tract-infection
Biofilm formation
Urethral catheters
Type-1 fimbriae
Spinal-cord
P-fimbriae
Expression
Strains
Strain-83972
Persistence
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2011 Collection
School of Chemistry and Molecular Biosciences
 
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Citation counts: TR Web of Science Citation Count  Cited 14 times in Thomson Reuters Web of Science Article | Citations
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Created: Sun, 18 Jul 2010, 10:05:31 EST