Increased transcription of Glutathione S-transferases in acaricide exposed scabies mites

Mounsey, Kate E., Pasay, Cielo J., Arlian, Larry G., Morgan, Marjorie S., Holt, Deborah C., Currie, Bart J., Walton, Shelley F. and McCarthy, James S. (2010) Increased transcription of Glutathione S-transferases in acaricide exposed scabies mites. Parasites and Vectors, 3 1: 43-1-43-9. doi:10.1186/1756-3305-3-43

Author Mounsey, Kate E.
Pasay, Cielo J.
Arlian, Larry G.
Morgan, Marjorie S.
Holt, Deborah C.
Currie, Bart J.
Walton, Shelley F.
McCarthy, James S.
Title Increased transcription of Glutathione S-transferases in acaricide exposed scabies mites
Journal name Parasites and Vectors   Check publisher's open access policy
ISSN 1756-3305
Publication date 2010-01-01
Sub-type Article (original research)
DOI 10.1186/1756-3305-3-43
Open Access Status DOI
Volume 3
Issue 1
Start page 43-1
End page 43-9
Total pages 9
Place of publication London, United Kingdom
Publisher BioMed Central
Language eng
Formatted abstract
Background: Recent evidence suggests that Sarcoptes scabiei var. hominis mites collected from scabies endemic communities in northern Australia show increasing tolerance to 5% permethrin and oral ivermectin. Previous findings have implicated detoxification pathways in developing resistance to these acaricides. We investigated the contribution of Glutathione S-transferase (GST) enzymes to permethrin and ivermectin tolerance in scabies mites using biochemical and molecular approaches.

Results: Increased in vitro survival following permethrin exposure was observed in S. scabiei var. hominis compared to acaricide naïve mites (p < 0.0001). The addition of the GST inhibitor diethyl maleate restored in vitro permethrin susceptibility, confirming GST involvement in permethrin detoxification. Assay of GST enzymatic activity in mites demonstrated that S. scabiei var. hominis mites showed a two-fold increase in activity compared to naïve mites (p < 0.0001). Increased transcription of three different GST molecules was observed in permethrin resistant S. scabiei var. canis- mu 1 (p < 0.0001), delta 1 (p < 0.001), and delta 3 (p < 0.0001). mRNA levels of GST mu 1, delta 3 and P-glycoprotein also significantly increased in S. scabiei var. hominis mites collected from a recurrent crusted scabies patient over the course of ivermectin treatment.

Conclusions: These findings provide further support for the hypothesis that increased drug metabolism and efflux mediate permethrin and ivermectin resistance in scabies mites and highlight the threat of emerging acaricide resistance to the treatment of scabies worldwide. This is one of the first attempts to define specific genes involved in GST mediated acaricide resistance at the transcriptional level, and the first application of such studies to S. scabiei, a historically challenging ectoparasite.
Keyword Sarcoptes scabiei
In vitro
Ivermectin resistance
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article number 43

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Medicine Publications
School of Veterinary Science Publications
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 28 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 33 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Sun, 18 Jul 2010, 10:02:21 EST