Proneural gene-linked neurogenesis in zebrafish cerebellum

Kani, S, Bae, YK, Shimizu, T, Tanabe, K, Satou, C, Parsons, MJ, Scott, E, Higashijima, S and Hibi, M (2010) Proneural gene-linked neurogenesis in zebrafish cerebellum. Developmental Biology, 343 1-2: 1-17. doi:10.1016/j.ydbio.2010.03.024

Author Kani, S
Bae, YK
Shimizu, T
Tanabe, K
Satou, C
Parsons, MJ
Scott, E
Higashijima, S
Hibi, M
Title Proneural gene-linked neurogenesis in zebrafish cerebellum
Journal name Developmental Biology   Check publisher's open access policy
ISSN 0012-1606
Publication date 2010-07-01
Year available 2010
Sub-type Article (original research)
DOI 10.1016/j.ydbio.2010.03.024
Open Access Status Not Open Access
Volume 343
Issue 1-2
Start page 1
End page 17
Total pages 17
Place of publication Maryland Heights, United States
Publisher Academic Press
Language eng
Formatted abstract
In mammals, cerebellar neurons are categorized as glutamatergic or GABAergic, and are derived from progenitors that express the proneural genes atoh1 or ptf1a, respectively. In zebrafish, three atoh1 genes, atoh1a, atoh1b, and atoh1c, are expressed in overlapping but distinct expression domains in the upper rhombic lip (URL): ptf1a is expressed exclusively in the ventricular zone (VZ). Using transgenic lines expressing fluorescent proteins under the control of the regulatory elements of atoh1a and ptf1a, we traced the lineages of the cerebellar neurons. The atoh1+ progenitors gave rise not only to granule cells but also to neurons of the anteroventral rhombencephalon. The ptf1a+ progenitors generated Purkinje cells. The olig2+ eurydendroid cells, which are glutamatergic, were derived mostly from ptf1a+ progenitors in the VZ but some originated from the atoh1+ progenitors in the URL. In the adult cerebellum, atoh1a, atoh1b, and atoh1c are expressed in the molecular layer of the valvula cerebelli and of the medial corpus cerebelli, and ptf1a was detected in the VZ. The proneural gene expression patterns coincided with the sites of proliferating neuronal progenitors in the adult cerebellum. Our data indicate that proneural gene-linked neurogenesis is evolutionarily conserved in the cerebellum among vertebrates, and that the continuously generated neurons help remodel neural circuits in the adult zebrafish cerebellum. © 2010 Elsevier Inc.
Keyword Zebrafish
Glutamatergic neuron
GABAergic neuron
Proneural gene
Adult neurogenesis
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID NCC-1010270
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Biomedical Sciences Publications
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Citation counts: TR Web of Science Citation Count  Cited 52 times in Thomson Reuters Web of Science Article | Citations
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Created: Sun, 04 Jul 2010, 10:05:39 EST