Characterization of the shsp genes in Drosophila buzzatii and association between the frequency of Valine mutations in hsp23 and climatic variables along a longitudinal gradient in Australia

Frydenberg, Jane, Barker, J. Stuart F. and Loeschcke, Volker (2010) Characterization of the shsp genes in Drosophila buzzatii and association between the frequency of Valine mutations in hsp23 and climatic variables along a longitudinal gradient in Australia. Cell Stress and Chaperones, 15 3: 271-280. doi:10.1007/s12192-009-0140-y

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Author Frydenberg, Jane
Barker, J. Stuart F.
Loeschcke, Volker
Title Characterization of the shsp genes in Drosophila buzzatii and association between the frequency of Valine mutations in hsp23 and climatic variables along a longitudinal gradient in Australia
Formatted title
Characterization of the shsp genes in Drosophila buzzatii and association between the frequency of Valine mutations in hsp23 and climatic variables along a longitudinal gradient in Australia
Journal name Cell Stress and Chaperones   Check publisher's open access policy
ISSN 1355-8145
1466-1268
Publication date 2010-05-01
Year available 2009
Sub-type Article (original research)
DOI 10.1007/s12192-009-0140-y
Volume 15
Issue 3
Start page 271
End page 280
Total pages 10
Editor Lawrence E. Hightower
Place of publication New York, NY, U.S.A.
Publisher Churchill Livingstone
Language eng
Subject 0601 Biochemistry and Cell Biology
C1
970106 Expanding Knowledge in the Biological Sciences
0604 Genetics
0603 Evolutionary Biology
Formatted abstract
The small heat shock gene (shsp) cluster of Drosophila buzzatii was sequenced and the gene order and DNA sequence were compared with those of the shsps in Drosophila melanogaster. The D. buzzatii shsp cluster contains an inversion and a duplication of hsp26. A phylogenetic tree was constructed based on hsp26 genes from several Drosophila species of the Sophophora and Drosophila subgenera. The tree shows first a separation of the Sophophora and the Drosophila subgenera and then the Drosophila subgenus is divided into the Hawaiian Drosophila and the repleta/virilis groups. Only the latter contain a duplicated hsp26. Comparing the gene organisation of the shsp cluster shows that all the Drosophila subgenus species contain the inversion. Putative heat shock elements (HSE) were found in the promoters of all the shsp and putative regulator elements for tissue specific expression were found in the promoter of hsp23, hsp27 and one of the hsp26 genes. hsp23 was found to be polymorphic for four non-synonymous changes that all lead to exchange of a Valine. The duplicated hsp26 gene in D. buzzatii (phsp26) was polymorphic for two non-synonymous changes. The allele frequencies of these variants were determined in nine D. buzzatii populations covering most of its distribution in Australia using high-resolution melting curves. The allele frequencies of one of the hsp23 variants showed a significant linear regression with longitude and the pooled frequency of the four Valine changes of hsp23 in the nine populations showed a significant linear regression with longitude and with a composite measure of climatic variables.
© 2009 Cell Stress Society International.
Keyword Heat shock protein
Gene duplication
Inversion
Conserved noncoding sequences
Geographical clines
Molecular population-genetics
Heat-shock
Microsatellite loci
New-world
Melanogaster
DNA
Sequence
Polymorphism
Elements
Patterns
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online: 6 October 2009. The online version of this article contains supplementary material, which is available to authorized users.

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
School of Biological Sciences Publications
 
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Created: Sun, 27 Jun 2010, 10:09:05 EST