Cytokine and chemokine gene expression profiles in heterophils from chickens treated with corticosterone

Shini, Shaniko, Shini, Agim and Kaiser, Pete (2010) Cytokine and chemokine gene expression profiles in heterophils from chickens treated with corticosterone. Stress, 13 3: 185-194. doi:10.3109/10253890903144639


Author Shini, Shaniko
Shini, Agim
Kaiser, Pete
Title Cytokine and chemokine gene expression profiles in heterophils from chickens treated with corticosterone
Journal name Stress   Check publisher's open access policy
ISSN 1025-3890
Publication date 2010-05-01
Sub-type Article (original research)
DOI 10.3109/10253890903144639
Volume 13
Issue 3
Start page 185
End page 194
Total pages 10
Place of publication Amsterdam, Netherlands
Publisher Harwood Academic Publishers
Collection year 2011
Language eng
Abstract In chickens, corticosterone is the end-product of stress. However, the nature of the immune response to elevated plasma corticosterone concentrations at the molecular level has not yet been characterised. We recently demonstrated that exposure to corticosterone in drinking water for 1 week significantly upregulates mRNA expression levels for the pro-inflammatory interleukins (IL)-1β, IL-6, IL-18 and the pro-inflammatory chemokine CCLi2 in chicken lymphocytes, particularly 3 h after the treatment started. In the present study, we investigated cytokine and chemokine mRNA expression levels in circulating heterophils of chickens, and show that at 3 h post initial treatment with corticosterone in drinking water (20 mg/1L) the mRNA expression levels for IL-1β, IL-6, IL-10, IL-12α and IL-18 are upregulated. The mRNA expression levels for IL-6, IL-10 and IL-18 correlate with plasma corticosterone concentration and total heterophil counts. Corticosterone downregulated the expression levels of all pro-inflammatory cytokines at 24 h and 1 week post-treatments. Repeated treatment with corticosterone upregulated mRNA expression levels of transforming growth factor-β4 and the chemokine CCL16. These data indicate that cytokine and chemokine gene expression signatures in chicken heterophils can be altered during stress and therefore could be used as an indicator of stress. Copyright © 2010 Informa Plc. All rights reserved.
Keyword Chemokine
Chicken
Corticosterone
Cytokine
Gene expression
Heterophil
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Agriculture and Food Sciences
 
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Created: Sun, 02 May 2010, 10:01:14 EST