Gut wash, body soak, blender and heat-fixation: Approaches to the effective collection, fixation and preservation of trematodes of fishes

Cribb, Thomas H. and Bray, Rodney A. (2010) Gut wash, body soak, blender and heat-fixation: Approaches to the effective collection, fixation and preservation of trematodes of fishes. Systematic Parasitology, 76 1: 1-7. doi:10.1007/s11230-010-9229-z


Author Cribb, Thomas H.
Bray, Rodney A.
Title Gut wash, body soak, blender and heat-fixation: Approaches to the effective collection, fixation and preservation of trematodes of fishes
Journal name Systematic Parasitology   Check publisher's open access policy
ISSN 0165-5752
1573-5192
Publication date 2010-05-01
Sub-type Article (original research)
DOI 10.1007/s11230-010-9229-z
Volume 76
Issue 1
Start page 1
End page 7
Total pages 7
Editor David I. Gibson
Place of publication The Hague, Netherlands
Publisher Kluwer
Language eng
Formatted abstract
Advice is offered on some effective methods for collecting and preserving trematodes from fishes for taxonomy and systematics. Emphasis is placed on obtaining high-quality specimens that have reliable data and that are amenable to study by both morphological and molecular approaches. We emphasise the importance of the freshness of the host specimen, the reliability of its provenance and the labelling of the specimens. For the collecting itself, we recommend a ‘gut-wash’ approach for gastro-intestinal species and specific searches for atypical taxa such as didymozoids, aporocotylids, Saturnius Manter, 1969 and transversotrematids. For metacercariae, we recommend a ‘blender’ approach to release parasites from host tissues. For fixation, we argue in favour of heat-killing in fluid at close to boiling temperature. We recommend against flattening as a routine procedure for collecting specimens for morphology. Preservation for morphological study is best in formalin or alcohol, and alcohol works well for molecular samples. The importance of reliable labelling and the deposition of specimens in museums is emphasised.
© 2010 Springer Science+Business Media B.V.
Keyword Gulf-of-mexico
Digenea
Australia
Sanguinicolidae
Perciformes
Queensland
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Chemistry and Molecular Biosciences
 
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Created: Sun, 02 May 2010, 10:01:10 EST